Two anaerobic membrane bioreactors (AnMBRs) equipped with different membrane pore size (0.4 or 0.05 μm) were operated at 25℃ and fed with domestic wastewater.The hydraulic retention time (HRT) of the reactors was shortened.The microbial communities of the two AnMBRs were investigated by 16S rRNA gene amplicon sequencing to see the effects of HRT.The predominant Archaea was an aceticlastic methanogen Methanosaeta.The composition of hydrogenotrophic methanogens changed with the HRTs:the population of Methanobae-terium was higher for longer HRTs,whereas the population of unclassified Methanoregu-laceae was higher for shorter HRTs.The Anaerolineae,Bacteroidia and Clostridia bacteria were dominant in both of the reactors,with a combined relative abundance of over 55％.The relative abundance of Anaerolineae was proportional to the biogas production performance.The change in the population of hydrogenotrophic methanogens or Anaerolineae can be used as an indicator for process monitoring.The sum of the relative abundance of Anaero-lineae and Clostridia fluctuated slightly with changes in the HRT in both AnMBRs when the reactor was stably operated.The co-occurrence analysis revealed the relative abundance of the operational taxonomic units belonging to Anaerolineae and Clostridia was functionally equivalent during the treatment of real domestic sewage.A principal coordination analysis revealed that the changes in the microbial community in each reactor were consistent with the change of HRT.In addition,both the HRT and the stability of the process are important factors for maintaining microbial community structures.

Jialing Ni;Jiayuan Ji;Yu-You Li;Kengo Kubota

Department of Civil and Environmental Engineering,Graduate School of Engineering,Tohoku University,6-6-06 Aoba,Aramaki,Aoba-ku 980-8579,Sendai,Japan;Department of Frontier Science for Advanced Environment,Graduate School of Environmental Studies,Tohoku University,6-6-06 Aoba,Aramaki,Aoba-ku 980-8579,Sendai,Japan

环境科学学报（英文版）

[1]Jialing Ni;Jiayuan Ji;Yu-You Li;Kengo Kubota-.Microbial characteristics in anaerobic membrane bioreactor treating domestic sewage:Effects of HRT and process performance)[J].环境科学学报（英文版）,2022(01):392-399

AnMBRs,aceticlastic,methanogen,hydrogenotrophic,HRTs,Methanobae,Methanoregu,Anaerolineae,lineae

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