Chlorine,chlorine dioxide,and ozone are widely used as disinfectants in drinking water treatments.However,the combined use of different disinfectants can result in the forma-tion of various organic and inorganic disinfection byproducts(DBPs).The toxic interactions,including synergism,addition,and antagonism,among the complex DBPs are still unclear.In this study,we established and verified a real-time cell analysis(RTCA)method for cy-totoxicity measurement on Chinese hamster ovary(CHO)cell.Using this convenient and accurate method,we assessed the cytotoxicity of a series of binary combinations consisting of one of the 3 inorganic DBPs(chlorite,chlorate,and bromate)and one of the 32 regulated and emerging organic DBPs.The combination index(CI)of each combination was calcu-lated and evaluated by isobolographic analysis to reflect the toxic interactions.The results confirmed the synergistic effect on cytotoxicity in the binary combinations consisting of chlorite and one of the 5 organic DBPs(2 iodinated DBPs(I-DBPs)and 3 brominated DBPs(Br-DBPs)),chlorate and one of the 4 organic DBPs(3 aromatic DBPs and dibromoacetoni-trile),and bromate and one of the 3 organic DBPs(2 I-DBPs and dibromoacetic acid).The possible synergism mechanism of organic DBPs on the inorganic ones may be attributed to the influence of organic DBPs on cell membrane and cell antioxidant system.This study re-vealed the toxic interactions among organic and inorganic DBPs,and emphasized the latent adverse outcomes in the combined use of different disinfectants.

Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology,School of Environmental Science and Engineering,Sun Yat-sen University,Guangzhou 510006,China

[1]Mengge Fan;Longfei Shu;Xinran Zhang;Miao Yu;Yongting Du;Junlang Qiu;Xin Yang-.Synergistic cytotoxicity of binary combinations of inorganic and organic disinfection byproducts assessed by real-time cell analysis)[J].环境科学学报（英文版）,2022(07):222-231

totoxicity,bromate,isobolographic,iodinated,dibromoacetoni,dibromoacetic

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