This study explored the antibiofilm efficacy of 3,3'-diindolylmethane (DIM) on Staphylococcus aureus and its disinfection on common food-contact surfaces. The minimum biofilm inhibitory concentration (MBIC) of DIM on S. aureus was 62.5 μmol/L, while it did not impede the bacterial growth evaluated by growth curve and XTT reduction assay. DIM in the concentration range of 31.2?62.5 μmol/L demonstrated a dose-dependent antibiofilm activity to S. aureus, as confirmed by light microscopic (LM), confocal laser scanning microscopic (CLSM), and scanning electron microscopic (SEM) analyses. At DIM of 62.5 μmol/L, the biomass of S. aureus biofilm was significantly reduced by 97％ and its average thickness by 58％ (P < 0.05). DIM of 62.5 μmol/L inhibited the bacterial initial adhesion and proliferation, as well as cell motility; the release of extracellular DNA (eDNA) and extracellular polysaccharide (EPS) were reduced by 75％ and 69％, respectively. DIM exhibited a strong inhibition to S. aureus biofilm formation on common food-contact surfaces, including 304 stainless steel, glass, and polyvinyl chloride (PVC) but not disperse the mature biofilm. Overall, our investigation identified DIM as a promising antibiofilm agent and its suitability to prevent the biofilm formation of S. aureus on common food-contact surfaces utilized during food processing.

Hui Zhang;Xiaomei Guo;Lei Tian;Na Wang;Yuqing Li;Ariel Kushmaro;Robert Marks;Qun Sun

College of Biomass Science and Engineering,Sichuan University,Chengdu 610064,China;Key Laboratory of Bio-resources and Eco-environment of the Ministry of Education,College of Life Sciences,Sichuan University,Chengdu 610064,China;Key Laboratory of Green Chemistry and Technology,Ministry of Education,College of Chemistry,Sichuan University,Chengdu 610064,China;State Key Laboratory of Oral Diseases&National Clinical Research Center for Oral Diseases&Department of Cariology and Endodontics,West China Hospital of Stomatology,Sichuan University,Chengdu 610065,China;Avram and Stella Goldstein-Goren Department of Biotechnology Engineering,Ben-Gurion University of the Negev,Beer-Sheva 84105,Israel;The Ilse Katz Center for Meso and Nanoscale Science and Technology,Ben-Gurion University of the Negev,Beer-Sheva 84105,Israel

食品科学与人类健康（英文）

[1]Hui Zhang;Xiaomei Guo;Lei Tian;Na Wang;Yuqing Li;Ariel Kushmaro;Robert Marks;Qun Sun-.Antibiofilm activity of 3,3'-diindolylmethane on Staphylococcus aureus and its disinfection on common food-contact surfaces)[J].食品科学与人类健康（英文）,2022(05):1222-1232

Antibiofilm

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