典型文献
DNA polymerase delta interacting protein 3 facilitates the activation and maintenance of DNA damage checkpoint in response to replication stress
文献摘要：
Background: Replication stress response is crucial for the maintenance of a stable ge-nome. POLDIP3 (DNA polymerase delta interacting protein 3) was initially identified as one of the DNA polymerase δ (Pol δ) interacting proteins almost 20 years ago. Using a variety of in vitro biochemical assays, we previously established that POLDIP3 is a key regulator of the enzymatic activity of Pol δ. However, the in vivo function of POLDIP3 in DNA replication and DNA damage response has been elusive. Methods: We first generated POLDIP3 knockout (KO) cells using the CRISPR/Cas9 technology. We then investigated its biological functions in vivo using a variety of biochemical and cell biology assays. Results: We showed that although the POLDIP3-KO cells manifest no pronounced defect in global DNA synthesis under nonstress conditions, they are sensitive to a va-riety of replication fork blockers. Intriguingly, we found that POLDIP3 plays a crucial role in the activation and maintenance of the DNA damage checkpoint in response to exogenous as well as endogenous replication stress. Conclusion: Our results indicate that when the DNA replication fork is blocked, POLDIP3 can be recruited to the stalled replication fork and functions to bridge the early DNA damage checkpoint response and the later replication fork repair/restart.
文献关键词：
中图分类号：
作者姓名：
Sufang Zhang;Ernest Y.C.Lee;Marietta Y.W.T.Lee;Dong Zhang
作者机构：
Department of Biochemistry and Molecular Biology,New York Medical College,New York,USA;Department of Biomedical Sciences,College of Osteopathic Medicine,New York Institute of Technology,New York,USA
文献出处：
引用格式：
[1]Sufang Zhang;Ernest Y.C.Lee;Marietta Y.W.T.Lee;Dong Zhang-.DNA polymerase delta interacting protein 3 facilitates the activation and maintenance of DNA damage checkpoint in response to replication stress)[J].动物模型与实验医学（英文）,2022(05):461-469
A类：
POLDIP3,nonstress
B类：
polymerase,delta,interacting,facilitates,activation,maintenance,damage,checkpoint,response,replication,Background,Replication,crucial,stable,nome,was,initially,identified,one,Pol,proteins,almost,years,ago,Using,variety,vitro,biochemical,assays,previously,established,that,key,regulator,enzymatic,activity,However,vivo,has,been,elusive,Methods,We,first,generated,knockout,KO,cells,using,CRISPR,Cas9,technology,then,investigated,its,biological,functions,biology,Results,showed,although,manifest,pronounced,defect,global,synthesis,under,conditions,they,are,sensitive,fork,blockers,Intriguingly,found,plays,role,exogenous,well,endogenous,Conclusion,Our,results,indicate,when,blocked,can,recruited,stalled,bridge,early,later,repair,restart
AB值：
0.490246
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