Objective:To investigate the effect of curcumol on NOD-like receptor thermoprotein domain 3(NLRP3)inflammasomes,and analyze the mechanism underlying curcumol against liver fibrosis.Methods:Thirty Kunming mice were divided into a control group,a model group and a curcumol group according to a random number table,10 mice in each group.Mice were intraperitoneally injected with 40％carbon tetrachloride(CCI4:peanut oil,2:3 preparation)at 5 mL/kg for 6 weeks,twice a week,for developing a liver fibrosis model.The mice in the control group were given the same amount of peanut oil,twice a week for 6 weeks.The mice in the curcumol group were given curcumol(30 mL/kg)intragastrically,and the mice in the model and control groups were given the same amount of normal saline,once a day for 6 weeks.Changes in liver structure were observed by hematoxylin and eosin(HE)and Masson staining.Liver function,liver fiber indices,and the expression of interleukin(IL)-10 and tumor necrosis factor-α(TNF-α)levels were determined by automatic biochemical analyzer and enzyme linked immunosorbent assay kit.Immunoblotting and reverse transcription-quantitative PCR(RT-qPCR)were performed to detect the expression of NLRP3 inflammasome-related molecules,TGF-β and collagen.Results:HE and Masson staining results showed that the hepatocytes of the model group were arranged irregularly with pseudo-lobular structure and a large amount of collagen deposition.The mice in the curcumol group had a significant decrease in liver function and liver fibers indices compared with the model group(P＜0.05);RT-qPCR and Western blot results reveal that,in the curcumol group,the mRNA and protein expression levels of NLRP3,IL-1 β,Caspase 1 and gasdermin D decreased significantly compared with the model group(P＜0.05);immunohistochemical results showed that in the curcumol group,the protein expression levels of NLRP3 and IL-1 β decreased significantly compared with the model group(P＜0.05).Conclusion:A potential anti-liver fibrosis mechanism of curcumol may be associated with the inhibition of NLRP3 inflammasomes and decreasing the downstream inflammatory response.

ZHENG Yang;WANG Lei;WANG Jia-hui;LIU Lu-lu;ZHAO Tie-jian

Department of Medicine,Faculty of Chinese Medicine Science,Guangxi University of Chinese Medicine,Nanning 530021,China;Department of Teaching,the First Affiliated Hospital of Guangxi University of Chinese Medicine,Nanning 530022,China;Department of Physiology,College of Basic Medicine,Guangxi University of Chinese Medicine,Nanning 530021,China

中国结合医学杂志（英文版）

[1]ZHENG Yang;WANG Lei;WANG Jia-hui;LIU Lu-lu;ZHAO Tie-jian-.Effect of Curcumol on NOD-Like Receptor Thermoprotein Domain 3 Inflammasomes in Liver Fibrosis of Mice)[J].中国结合医学杂志（英文版）,2022(11):992-999

Curcumol,Thermoprotein,Inflammasomes,curcumol,thermoprotein

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