Marine organisms cannot grow and reproduce without proper metabolic regulation.Within a metabolic network,problems with a given link will affect the normal life activities of the organism.Many metabolic mechanisms associated with behaviors of Am-phioctopus fangsiao are still unclear.Moreover,as a factor affecting the normal growth of A.fangsiao,egg protection has rarely been considered in previous behavioral studies.In this research,we analyzed the transcriptome profile of gene expression in A.fangsiao egg-unprotected larvae and egg-protected larvae,and identified 818 differentially expressed genes(DEGs).We used GO and KEGG enrichment analyses to search for metabolism-related DEGs.Protein-protein interaction networks were constructed to examine the interactions between metabolism-related genes.Twenty hub genes with multiple protein-protein interaction relationships or that were involved in multiple KEGG signaling pathways were obtained and verified by quantitative RT-PCR.We first studied the effects of egg protection on the metabolism of A.fangsiao larvae by means of protein-protein interaction networks,and the results provide va-luable gene resources for understanding the metabolism of invertebrate larvae.The data serve as a foundation for further research on the egg-protecting behavior of invertebrates.

BAO Xiaokai;LI Zan;ZHANG Jianbai;LI Yan;CHEN Xipan;WANG Weijun;SUN Guohua;XU Xiaohui;LIU Xiumei;FENG Yanwei;YANG Jianmin

School of Agriculture,Ludong University,Yantai 264025,China;Yantai Marine Economic Research Institute,Yantai 264004,China;College of Life Sciences,Yantai University,Yantai 264005,China

中国海洋大学学报（自然科学英文版）

[1]BAO Xiaokai;LI Zan;ZHANG Jianbai;LI Yan;CHEN Xipan;WANG Weijun;SUN Guohua;XU Xiaohui;LIU Xiumei;FENG Yanwei;YANG Jianmin-.Transcriptome Profile Based on Protein-Protein Interaction Networks Provides a Set of Core Genes for Understanding the Metabolic Mechanisms of the Egg-Protecting Behavior in Amphioctopus fangsiao)[J].中国海洋大学学报（自然科学英文版）,2022(05):1323-1333

Amphioctopus,phioctopus,luable

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