Peptidoglycan recognition protein(PGRP)plays a vital role in invertebrate innate immunity system as a specific pattern recognition receptor for peptidoglycan.Bivalves possess various PGRP systems for self-defense;however,it has not been characterized in razor clam Sinonovacula constricta.In this study,eight PGRP coding sequences were identified and analyzed from S.constricta genome,which are designated as ScPGRP-S1,ScPGRP-S2,ScPGRP-S3,ScPGRP-S4,ScPGRP-S5,ScPGRP-S6,ScPGRP-S7,ScPGRP-S8.The results of molecular evolutionary analyses showed that all eight ScPGRP genes were highly conserved and exhi-bited a typical PGRP/amidase 2 domain as PGRP genes in other mollusks.Moreover,the presence of signal peptides was predicted in ScPGRP-S2,ScPGRP-S3 and ScPRP-S6,while a transmembrane structure only existed in ScPGRP-S6.Notably,a tertiary struc-ture analysis indicated that no disulfide bond was observed in ScPGRP-S5 and ScPGRP-S7.The mRNA transcripts analysis of ScPGRPs revealed that the high expression patterns of ScPGRP-S1 and ScPGRP-S4 were found in mantle,adductor muscle and foot,while those of ScPGRP-S2,ScPGRP-S3 and ScPGRP-S6 were observed in hepatopancreas.Furthermore,the temporal expression profiles of ScPGRPs in the hepatopancreas were analyzed by qPCR after Gram-negative Vibrio parahaemolyticus and Gram-positive Staphylococcus aureus challenges.The mRNA expressions of ScPGRP-S2,ScPGRP-S3 and ScPGRP-S6 could be induced by V.pa-rahaemolyticus and S.aureus.Overall,our findings indicated that ScPGRPs were involved in the immune defense against invaders,which constituted a comprehensive understanding of the potential role of PGRP genes in S.constricta.

MENG Yiping;LU Liyuan;DAI Wenfang;ZHANG Weiwei;LIN Zhihua;DONG Yinghui

School of Marine Sciences,Ningbo University,Ningbo 315010,China;Zhejiang Key Laboratory of Aquatic Germplasm Resource,College of Biological&Environmental Sciences,Zhejiang Wanli University,Ningbo 315100,China;Ninghai Institute of Mariculture Breeding and Seed Industry,Zhejiang Wanli University,Ninghai 315604,China

中国海洋大学学报（自然科学英文版）

[1]MENG Yiping;LU Liyuan;DAI Wenfang;ZHANG Weiwei;LIN Zhihua;DONG Yinghui-.Identification,Phylogeny and Expressional Profiles of Peptidoglycan Recognition Protein(PGRP)Gene Family in Sinonovacula constricta)[J].中国海洋大学学报（自然科学英文版）,2022(04):1051-1060

Expressional,Peptidoglycan,Bivalves,razor,ScPGRP,bited,amidase,ScPRP,ScPGRPs,rahaemolyticus

Identification,Phylogeny,Profiles,Recognition,Protein,Gene,Family,Sinonovacula,constricta,recognition,protein,plays,vital,role,invertebrate,innate,immunity,specific,receptor,peptidoglycan,possess,various,systems,self,defense,however,has,not,been,characterized,clam,In,this,study,eight,coding,sequences,were,identified,analyzed,from,genome,which,are,designated,S1,S2,S3,S4,S5,S6,S7,S8,results,molecular,evolutionary,analyses,showed,that,genes,highly,conserved,exhi,typical,domain,other,mollusks,Moreover,presence,signal,peptides,was,predicted,while,transmembrane,structure,only,existed,Notably,tertiary,analysis,indicated,disulfide,bond,observed,transcripts,revealed,patterns,found,mantle,adductor,muscle,foot,those,hepatopancreas,Furthermore,temporal,profiles,by,qPCR,after,Gram,negative,Vibrio,parahaemolyticus,positive,Staphylococcus,aureus,challenges,expressions,could,induced,Overall,our,findings,involved,immune,against,invaders,constituted,comprehensive,understanding,potential

0.442715