典型文献
MicroRNA-101a-3p mimic ameliorates spinal cord ischemia/reperfusion injury
文献摘要：
miR-101a-3p is expressed in a variety of organs and tissues and plays a regulatory role in many diseases,but its role in spinal cord ischemia/reperfusion injury remains unclear.In this study,we established a rat model of spinal cord ischemia/reperfusion injury by clamping the aortic arch for 14 minutes followed by reperfusion for 24 hours.Results showed that miR-101a-3p expression in L4-L6 spinal cord was greatly decreased,whereas MYCN expression was greatly increased.Dual-luciferase reporter assay results showed that miR-101a-3p targeted MYCN.MYCN immunoreactivity,which was primarily colocalized with neurons in L4-L6 spinal tissue,greatly increased after spinal cord ischemia/reperfusion injury.However,intrathecal injection of an miR-101a-3p mimic within 24 hours before injury decreased MYCN,p53,caspase-9 and interleukin-1β expression,reduced p53 immunoreactivity,reduced the number of MYCN/NeuN-positive cells and the number of necrotic cells in L4-L6 spinal tissue,and increased Tarlov scores.These findings suggest that the miR-101a-3p mimic improved spinal ischemia/reperfusion injury-induced nerve cell apoptosis and inflammation by inhibiting MYCN and the p53 signaling pathway.Therefore,miR-101a-3p mimic therapy may be a potential treatment option for spinal ischemia/reperfusion injury.
文献关键词：
中图分类号：
作者姓名：
Zai-Li Zhang;Dan Wang;Feng-Shou Chen
作者机构：
Department of Anesthesiology,the First Hospital of China Medical University,Shenyang,Liaoning Province,China
文献出处：
引用格式：
[1]Zai-Li Zhang;Dan Wang;Feng-Shou Chen-.MicroRNA-101a-3p mimic ameliorates spinal cord ischemia/reperfusion injury)[J].中国神经再生研究（英文版）,2022(09):2022-2028
A类：
101a,intrathecal
B类：
MicroRNA,3p,mimic,ameliorates,spinal,cord,ischemia,reperfusion,injury,miR,expressed,variety,organs,tissues,plays,regulatory,role,many,diseases,but,its,remains,unclear,In,this,study,established,model,by,clamping,aortic,arch,minutes,followed,hours,Results,showed,that,expression,L4,L6,was,greatly,decreased,whereas,MYCN,increased,Dual,luciferase,reporter,assay,results,targeted,immunoreactivity,which,primarily,colocalized,neurons,after,However,injection,within,before,p53,caspase,interleukin,reduced,number,NeuN,positive,cells,necrotic,Tarlov,scores,These,findings,suggest,improved,induced,nerve,apoptosis,inflammation,inhibiting,signaling,pathway,Therefore,therapy,may,potential,treatment,option
AB值：
0.457649
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