Previous studies have shown that vagus nerve stimulation can improve patients' locomotor function. The stimulation of the auricular vagus nerve, which is the only superficial branch of the vagus nerve, may have similar effects to vagus nerve stimulation. However, the precise mechanisms remain poorly understood. In this study, rat models of cerebral ischemia/reperfusion injury were established by modified Longa ligation. Twenty-four hours later, 7-day auricular vagus nerve stimulation was performed. The results showed that auricular vagus nerve stimulation promoted the secretion of acetylcholine, inhibited the secretion of interleukin-1β, interleukin-6, and tumor necrosis factor-α, and reduced connexin 43 phosphorylation in the ischemic penumbra and motor cortex, promoting locomotor function recovery in rats with cerebral ischemia/reperfusion injury. These findings suggested that auricular vagus nerve stimulation promotes the recovery of locomotor function in rats with cerebral ischemia/reperfusion injury by altering the secretion of acetylcholine and inflammatory factors and the phosphorylation of connexin 43. This study was approved by the Animal Use and Management Committee of Shanghai University of Traditional Chinese Medicine on November 8, 2019 (approval No. PZSHUTCM191108014).

Center of Rehabilitation Medicine,Yueyang Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of Traditional ChineseMedicine,Shanghai,China;School of Rehabilitation Science,Shanghai University of Traditional Chinese Medicine,Shanghai,China;Engineering ResearchCenter of Traditional Chinese Medicine Intelligent Rehabilitation,Ministry of Education,Shanghai,China;Center of Rehabilitation Medicine,the First Affiliated Hospital of Xinxiang Medical University,Xinxiang,Henan Province,China;Shanghai Research Institute of Acupuncture and Meridian,Shanghai,China;Institute of Acupuncture and Moxibustion,Chinese Academy of Chinese Medical Sciences,Beijing,China

[1]Jing-Jun Zhao-.The mechanisms through which auricular vagus nerve stimulation protects against cerebral ischemia/reperfusion injury)[J].中国神经再生研究（英文版）,2022(03):594-600

PZSHUTCM191108014

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