典型文献
Downregulation of EphB2 by RNA interference attenuates glial/fibrotic scar formation and promotes axon growth
文献摘要：
The rapid formation of a glial/fibrotic scar is one of the main factors hampering axon growth after spinal cord injury. The bidirectional EphB2/ephrin-B2 signaling of the fibroblast-astrocyte contact-dependent interaction is a trigger for glial/fibrotic scar formation. In the present study, a new in vitro model was produced by coculture of fibroblasts and astrocytes wounded by scratching to mimic glial/fibrotic scar-like structures using an improved slide system. After treatment with RNAi to downregulate EphB2, changes in glial/fibrotic scar formation and the growth of VSC4.1 motoneuron axons were examined. Following RNAi treatment, fibroblasts and astrocytes dispersed without forming a glial/fibrotic scar-like structure. Furthermore, the expression levels of neurocan, NG2 and collagen I in the coculture were reduced, and the growth of VSC4.1 motoneuron axons was enhanced. These findings suggest that suppression of EphB2 expression by RNAi attenuates the formation of a glial/fibrotic scar and promotes axon growth. This study was approved by the Laboratory Animal Ethics Committee of Jiangsu Province, China (approval No. 2019-0506-002) on May 6, 2019.
文献关键词：
中图分类号：
作者姓名：
Jian Wu
作者机构：
Department of Histology and Embryology,Medical College,Nantong University,Nantong,Jiangsu Province,China
文献出处：
引用格式：
[1]Jian Wu-.Downregulation of EphB2 by RNA interference attenuates glial/fibrotic scar formation and promotes axon growth)[J].中国神经再生研究（英文版）,2022(02):362-369
A类：
VSC4,motoneuron,neurocan
B类：
Downregulation,EphB2,by,interference,attenuates,glial,fibrotic,scar,formation,promotes,growth,rapid,main,factors,hampering,after,spinal,cord,injury,bidirectional,ephrin,signaling,contact,dependent,interaction,trigger,In,present,study,new,vitro,model,was,produced,coculture,fibroblasts,astrocytes,wounded,scratching,mimic,like,structures,using,improved,slide,system,After,treatment,RNAi,downregulate,changes,axons,were,examined,Following,dispersed,without,forming,Furthermore,expression,levels,NG2,collagen,reduced,enhanced,These,findings,suggest,that,suppression,This,approved,Laboratory,Animal,Ethics,Committee,Jiangsu,Province,China,approval,No,May
AB值：
0.481809
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机标中图分类号，由域田数据科技根据网络公开资料自动分析生成，仅供学习研究参考。
