典型文献
CRISPR/Cas9-Targeted Knockout of Rice Susceptibility Genes OsDjA2 and OsERF104 Reveals Alternative Sources of Resistance to Pyricularia oryzae
文献摘要：
Rice genes OsDjA2 and OsERF104,encoding a chaperone protein and an APETELA2/ethylene-responsive factor,respectively,are strongly induced in a compatible interaction with blast fungus,and also have function in plant susceptibility validated through gene silencing.Here,we reported the CRISPR/Cas9 knockout of OsDjA2 and OsERF104 genes resulting in considerable improvement of blast resistance.A total of 15 OsDjA2(62.5％)and 17 OsERF104(70.8％)T0 transformed lines were identified from 24 regenerated plants for each target and used in downstream experiments.Phenotyping of homozygous T1 mutant lines revealed not only a significant decrease in the number of blast lesions but also a reduction in the percentage of diseased leaf area,compared with the infected control plants.Our results supported CRISPR/Cas9-mediated target mutation in rice susceptibility genes as a potential and alternative breeding strategy for building resistance to blast disease.
文献关键词：
中图分类号：
作者姓名：
Fabiano T.P.K.TáVORA;Anne Cécile MEUNIER;Aurore VERNET;Murielle PORTEFAIX;Jo?lle MILAZZO;Henri ADREIT;Didier THARREAU;Octávio L.FRANCO;Angela MEHTA
作者机构：
Department of Genetics and Biotechnology,Federal University of Juiz de Fora,Juiz de Fora-MG 36036330,Brazil;Embrapa Genetic Resources and Biotechnology,Brasilia-DF 70770-917,Brazil;Genetic Improvement and Adaptation of Mediterranean and Tropical Plants,Agricultural Research Center for International Development(CIRAD),Montpellier 34398 CEDEX 5,France;CIRAD-INRAe-Agro Institute,University of Montpellier,Montpellier 34000,France;Plant Health Institute of Montpellier,CIRAD,Montpellier 34398,France;University of Montpellier,National Institute of Agronomic Research,Research Institute for Development,Montpellier SupAgro,Montpellier 34398,France;Department of Genomics Science and Biotechnology,Proteomics Analysis Center,Catholic University of Brasilia,Brasilia-DF 71966-900,Brazil;S-Inova Biotech,Catholic University of Dom Bosco,Campo Grande-MS 79117-900,Brazil
文献出处：
引用格式：
[1]Fabiano T.P.K.TáVORA;Anne Cécile MEUNIER;Aurore VERNET;Murielle PORTEFAIX;Jo?lle MILAZZO;Henri ADREIT;Didier THARREAU;Octávio L.FRANCO;Angela MEHTA-.CRISPR/Cas9-Targeted Knockout of Rice Susceptibility Genes OsDjA2 and OsERF104 Reveals Alternative Sources of Resistance to Pyricularia oryzae)[J].水稻科学（英文版）,2022(06):535-544,中插22-中插29
A类：
OsDjA2,OsERF104,APETELA2
B类：
CRISPR,Cas9,Targeted,Knockout,Rice,Susceptibility,Genes,Reveals,Alternative,Sources,Resistance,Pyricularia,oryzae,genes,encoding,chaperone,protein,ethylene,responsive,respectively,strongly,induced,compatible,interaction,blast,fungus,also,have,function,susceptibility,validated,through,silencing,Here,reported,knockout,resulting,considerable,improvement,resistance,total,T0,transformed,lines,were,identified,from,regenerated,plants,each,target,used,downstream,experiments,Phenotyping,homozygous,mutant,revealed,only,significant,decrease,number,lesions,but,reduction,percentage,diseased,leaf,area,compared,infected,control,Our,results,supported,mediated,mutation,rice,potential,alternative,breeding,strategy,building
AB值：
0.607506
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