Understanding the impact of biological activities on the soil phosphorus (P) distribution under long-term fertilizer application can facilitate better soil P fertility management. Therefore, the primary objectives of this study were to investigate the effect of long-term (since 1981) fertilizer application on the soil P fractions and microbial community and to evaluate correlations between the microbial community structure and P distribution. The following treatments were implemented in a long-term field trial: no fertilization (CK), inorganic N and K (NK), inorganic P and K (PK), inorganic N, P and K (NPK) and manure+NPK (MNPK) fertilization. The study showed that the soil pH, soil organic carbon and total and available N and P concentrations were considerably higher in the MNPK treatment than in the CK treatment. The soil microbial biomass C, N and P concentrations were also significantly higher in the MNPK treatment than in the CK treatment. Among fertilization treatments, the β-1,4-glucosidase, α-1,4-glucosidase, urease, acid phosphatase and phosphodiesterase activities were the highest in the MNPK treatment. Compared to inorganic fertilization, the MNPK treatment increased the labile soil P fractions and decreased the residual soil P concentration. Continuous fertilization significantly affected the soil microbial composition. The total phospholipid fatty acid (PLFA) concentrations in the NK, PK, NPK and MNPK treatments were 23.3, 43.1, 48.7 and 87.7％ higher, respectively, than in the CK treatment. A significant correlation was observed between the microbial community and soil P fractions. Moreover, the aggregated boosted tree (ABT) model showed that among the various soil biochemical properties, the total PLFA concentration was the factor that most influenced the active P pool, accounting for 35.4％ of the relative influence of all soil biochemical properties examined. These findings reveal that combined manure and inorganic fertilizer application is a better approach than applying inorganic fertilizer alone for sustaining long-term P fertility by mediating soil biological activity.

Muhammad QASWAR;Waqas AHMED;HUANG Jing;LIU Kai-lou;ZHANG Lu;HAN Tian-fu;DU Jiang-xue;Sehrish ALI;Hafeez UR-RAHIM;HUANG Qing-hai;ZHANG Hui-min

National Engineering Laboratory for Improving Quality of Arable Land/Institute of Agricultural Resources and Regional Planning,Chinese Academy of Agricultural Sciences,Beijing 100081,P.R.China;Department of Environment,Faculty of Bioscience Engineering,Ghent University,Ghent 9000,Belgium;Guangdong Provincial Key Laboratory for Radionuclides Pollution Control and Resources,School of Environmental Science and Engineering,Guangzhou University,Guangzhou 510006,P.R.China;National Observation Station of Qiyang Agri-ecology System/Institute of Agricultural Resources and Regional Planning,Chinese Academy of Agricultural Sciences,Qiyang 426182,P.R.China;National Engineering and Technology Research Center for Red Soil Improvement/Jiangxi Institute of Red Soil,Nanchang 331717,P.R.China;Key Laboratory of Industrial Ecology and Environmental Engineering,Ministry of Education/School of Environmental Science and Technology,Dalian University of Technology,Dalian 116024,P.R.China

农业科学学报（英文）

[1]Muhammad QASWAR;Waqas AHMED;HUANG Jing;LIU Kai-lou;ZHANG Lu;HAN Tian-fu;DU Jiang-xue;Sehrish ALI;Hafeez UR-RAHIM;HUANG Qing-hai;ZHANG Hui-min-.Interaction of soil microbial communities and phosphorus fractions under long-term fertilization in paddy soil)[J].农业科学学报（英文）,2022(07):2134-2144

manure+NPK

Interaction,soil,microbial,communities,phosphorus,fractions,under,long,term,fertilization,paddy,Understanding,impact,biological,activities,distribution,fertilizer,application,facilitate,better,fertility,management,Therefore,primary,objectives,this,study,were,investigate,effect,since,community,evaluate,correlations,between,structure,following,treatments,implemented,field,trial,inorganic,NK,MNPK,showed,that,carbon,total,available,concentrations,considerably,higher,than,biomass,also,significantly,Among,glucosidase,urease,acid,phosphatase,phosphodiesterase,highest,Compared,increased,labile,decreased,residual,Continuous,affected,composition,phospholipid,fatty,PLFA,respectively,was,observed,Moreover,aggregated,boosted,tree,ABT,model,among,various,biochemical,properties,most,influenced,active,pool,accounting,relative,all,examined,These,findings,reveal,combined,approach,applying,alone,sustaining,by,mediating,activity

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