典型文献
PbPH5, an H+ P-ATPase on the tonoplast, is related to malic acid accumulation in pear fruit
文献摘要：
Organic acid content is one of the most important factors influencing fruit flavors. The predominant organic acid in most pear cultivars is malic acid, but the mechanism controlling its accumulation remains unclear. In this study, by comparing gene expression levels and organic acid contents, we found that the expression of PbPH5, which encodes a P3A-ATPase, is highly correlated with malic acid accumulation in four different pear species, with correlation coefficients of 0.932**, 0.656*, 0.900**, and 0.518* (*, P<0.05; **, P<0.01) for Pyrus bretschneideri Rehd., P. communis Linn., P. pyrifolia Nakai., and P. ussuriensis Maxim., respectively. Moreover, the overexpression of PbPH5 in pear significantly increased the malic acid content. In contrast, silencing PbPH5 via RNA interference significantly decreased both its transcript level and the pear fruit malic acid content. A subcellular localization analysis indicated that PbPH5 is located in the tonoplast. Additionally, a phylogenetic analysis indicated that PbPH5 is a PH5 homolog gene that is clustered with the Petunia hybrida, Malus domestica, and Citrus reticulata genes. Considered together, these findings suggest that PbPH5 is a functionally conserved gene. Furthermore, the accumulation of malic acid in pear fruit is at least partly related to changes in the PbPH5 transcription levels.
文献关键词：
中图分类号：
作者姓名：
SONG Jun-xing;CHEN Ying-can;LU Zhao-hui;ZHAO Guang-ping;WANG Xiao-li;ZHAI Rui;WANG Zhi-gang;YANG Cheng-quan;XU Ling-fei
作者机构：
College of Horticulture,Northwest A&F University,Yangling 712100,P.R.China
文献出处：
引用格式：
[1]SONG Jun-xing;CHEN Ying-can;LU Zhao-hui;ZHAO Guang-ping;WANG Xiao-li;ZHAI Rui;WANG Zhi-gang;YANG Cheng-quan;XU Ling-fei-.PbPH5, an H+ P-ATPase on the tonoplast, is related to malic acid accumulation in pear fruit)[J].农业科学学报（英文）,2022(06):1645-1657
A类：
PbPH5,P3A,pyrifolia,PH5,Petunia
B类：
H+,ATPase,tonoplast,malic,acid,accumulation,pear,fruit,Organic,one,most,important,factors,influencing,flavors,predominant,organic,cultivars,but,mechanism,controlling,its,remains,unclear,In,this,study,by,comparing,levels,contents,we,found,that,which,encodes,highly,correlated,four,different,species,correlation,coefficients,Pyrus,bretschneideri,Rehd,communis,Linn,Nakai,ussuriensis,Maxim,respectively,Moreover,overexpression,significantly,increased,contrast,silencing,via,interference,decreased,both,subcellular,localization,analysis,indicated,located,Additionally,phylogenetic,homolog,clustered,hybrida,Malus,domestica,Citrus,reticulata,genes,Considered,together,these,findings,suggest,functionally,conserved,Furthermore,least,partly,changes,transcription
AB值：
0.485098
相似文献
机标中图分类号，由域田数据科技根据网络公开资料自动分析生成，仅供学习研究参考。
