典型文献
Overexpression of Brassica napus cytosolic fructose-1,6-bisphosphatase and sedoheptulose-1,7-bisphosphatase genes significantly enhanced tobacco growth and biomass
文献摘要：
Elevated activities of cytosolic fructose-1,6-bisphosphatase (cyFBPase) and sedoheptulose-1,7-bisphosphatase (SBPase) are associated with higher yields in plants. In this study, the expression levels of the cyFBPase and SBPase genes were increased by overexpressing rape (Brassica napus) cDNA in tobacco (Nicotiana tabacum) plants. The transgenic plants co-expressing cyFBPase and SBPase (TpFS), or expressing single cyFBPase (TpF) or SBPase (TpS) had 1.77-, 1.55-, 1.23-fold cyFBPase and 1.45-, 1.12-, 1.36-fold SBPase activities as compared to the wild-type (WT), respectively. Photosynthesis rates of TpF, TpS and TpFS increased 4, 20 and 25％ compared with WT plants. The SBPase and cyFBPase positively regulated each other and functioned synergistically in transgenic tobacco plants. In addition, the sucrose contents of the three transgenic plants were higher than that of WT plants. The starch accumulation of the TpFS and TpS plants was improved by 53 and 37％, but slightly decreased in TpF plants. Moreover, the transgenic tobacco plants harbouring SBPase and/or cyFBPase genes showed improvements in their growth, biomass, dry weight, plant height, stem diameter, leaf size, flower number, and pod weight. In conclusion, co-expression of SBPase and cyFBPase may pave a new way for improving crop yield in agricultural applications.
文献关键词：
中图分类号：
作者姓名：
LI Yan-yan;GUO Li-na;LIANG Cheng-zhen;MENG Zhi-gang;Syed TAHIRA;GUO San-dui;ZHANG Rui
作者机构：
Biotechnology Research Institute,Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement,Beijing 100081,P.R.China
文献出处：
引用格式：
[1]LI Yan-yan;GUO Li-na;LIANG Cheng-zhen;MENG Zhi-gang;Syed TAHIRA;GUO San-dui;ZHANG Rui-.Overexpression of Brassica napus cytosolic fructose-1,6-bisphosphatase and sedoheptulose-1,7-bisphosphatase genes significantly enhanced tobacco growth and biomass)[J].农业科学学报（英文）,2022(01):49-59
A类：
sedoheptulose,cyFBPase,SBPase,TpFS,TpF,TpS,harbouring
B类：
Overexpression,Brassica,napus,cytosolic,fructose,bisphosphatase,genes,significantly,enhanced,tobacco,growth,biomass,Elevated,activities,associated,higher,yields,plants,In,this,study,levels,were,increased,by,overexpressing,rape,cDNA,Nicotiana,tabacum,transgenic,single,had,fold,compared,wild,type,WT,respectively,Photosynthesis,rates,positively,regulated,each,other,functioned,synergistically,addition,sucrose,contents,three,than,that,starch,accumulation,was,improved,but,slightly,decreased,Moreover,showed,improvements,their,dry,weight,height,stem,diameter,leaf,size,flower,number,pod,conclusion,may,pave,new,way,improving,crop,agricultural,applications
AB值：
0.396841
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