M acrobrachium nipponense is an economically important freshwater prawn that is often threatened by many aquatic pathogens. In this study, comparative transcriptomic analysis was firstly used to explore the transcriptional response of M . nipponense to Aeromonas veronii or Staphylococcus aureus stimulation. A total of 400.19 million clean reads were obtained and assembled into 56944 unigenes with an average length of 1253 bp. A total of 1857 differentially expressed genes were found after A . veronii infection, including 677 genes that were up-regulated and 1180 genes that were down-regulated, while 1061 significant differentially expressed genes were identified after S . aureus infection, including 390 up-regulated and 671 down-regulated genes. Many immune-related genes including Spaetzle, prophenoloxidase activating factor, C-type lectin, anti-lipopolysaccharide factor, and inhibitor of apoptosis 2 protein were commonly up-regulated after A . veronii or S . aureus infection. This study will enrich our understanding of the immune response to gram-positive and gram-negative bacteria infection in crustaceans.

Chong HAN;Zhipeng ZHANG;Qiang LI;Qinghua CHEN;Jianrong HUANG

State Key Laboratory of Biocontrol,Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals,School of life Sciences,Sun Yat-Sen University,Guangzhou 510275,China;School of life Sciences,Guangzhou University,Guangzhou 510006,China;South China Institute of Environmental Science,MEE,Guangzhou 510610,China;Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai),Zhuhai 519082,China

海洋湖沼学报（英文版）

[1]Chong HAN;Zhipeng ZHANG;Qiang LI;Qinghua CHEN;Jianrong HUANG-.Comparative transcriptomic analysis of Macrobrachium nippon ense in response to Aeromonas veronii or Staphylococcus aureus infection)[J].海洋湖沼学报（英文版）,2022(01):347-359

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