典型文献
Localization of urea transporter B in the developing bovine rumen
文献摘要：
Urea nitrogen secreted from blood to rumen is a crucial factor shaping the symbiotic relationship be-tween host ruminants and their microbial populations.Passage of urea across rumen epithelia is facil-itated by urea transporter B(UT-B),but the long-term regulation of these proteins remains unclear.As ruminal function develops over a period of months,the developing rumen is an excellent model with which to investigate this regulation.Using rumen epithelium samples of calves from birth to 96 d of age,this study performed immunolocalization studies to localize and semi-quantify UT-B protein develop-ment.As expected,preliminary experiments confirmed that ruminal monocarboxylate transporter 1(MCT1)short chain fatty acid transporter protein abundance increased with age(P＜0.01,n=4).Further investigation revealed that ruminal UT-B was present in the first few weeks of life and initially detected in the basolateral membrane of stratum basale cells.Over the next 2 months,UT-B staining spread to other epithelial layers and semi-quantification indicated that UT-B abundance significantly increased with age(P＜0.01,n=4 or 6).These changes were in line with the development of rumen function after the advent of solid feed intake and weaning,exhibiting a similar pattern to both MCT1 transporters and papillae growth.This study therefore confirmed age-dependent changes of in situ ruminal UT-B protein,adding to our understanding of the long-term regulation of ruminal urea transporters.
文献关键词：
中图分类号：
作者姓名：
Chongliang Zhong;Tamsin Lyons;Orla Heussaff;Evelyn Doyle;Eoin O'Hara;Sinead M.Waters;David Kenny;Gavin S.Stewart
作者机构：
School of Biology and Environmental Science,University College Dublin,Belfield,Dublin 4,Ireland;Animal and Bioscience Research Department,Animal and Grassland Research and Innovation Centre,Teagasc,Grange,County Meath,Ireland;Department of Agriculture,Food,and Nutritional Sciences,University of Alberta,Edmonton,Alberta,Canada
文献出处：
引用格式：
[1]Chongliang Zhong;Tamsin Lyons;Orla Heussaff;Evelyn Doyle;Eoin O'Hara;Sinead M.Waters;David Kenny;Gavin S.Stewart-.Localization of urea transporter B in the developing bovine rumen)[J].动物营养（英文）,2022(03):216-222
A类：
itated,calves,immunolocalization,basale
B类：
Localization,urea,developing,bovine,rumen,Urea,nitrogen,secreted,from,blood,crucial,shaping,symbiotic,relationship,be,tween,host,ruminants,their,microbial,populations,Passage,across,facil,by,UT,but,long,term,regulation,these,proteins,remains,unclear,ruminal,function,develops,over,period,months,excellent,model,which,investigate,this,Using,epithelium,samples,birth,study,performed,studies,localize,semi,quantify,expected,preliminary,experiments,confirmed,that,monocarboxylate,MCT1,short,chain,fatty,acid,abundance,increased,Further,investigation,revealed,was,present,first,few,weeks,life,initially,detected,basolateral,membrane,stratum,cells,Over,next,staining,spread,other,epithelial,layers,quantification,indicated,significantly,These,changes,were,line,development,after,advent,solid,feed,intake,weaning,exhibiting,similar,pattern,both,transporters,papillae,growth,This,therefore,dependent,situ,adding,our,understanding
AB值：
0.574132
相似文献
机标中图分类号，由域田数据科技根据网络公开资料自动分析生成，仅供学习研究参考。
