A microscale vaccine containing SiO2 nanoparticles loaded in CaCO3 microparticles was constructed using the co-precipitation method.The antigen ovalbumin(OVA)was covalently conjugated with SiO2 nanopar-ticles,and these nanoparticles and CpG were co-encapsulated into CaCO3 microparticles,generating a vaccine with a size of approximately 5.2 μm.Scanning electron microscopy(SEM),energy-dispersive X-ray(EDX),elemental mapping,and Fourier transform infrared(FTIR)analyses confirmed the successful preparation of the microscale vaccine;the vaccine had good storage stability without sustained anti-gen release,and negligible cytotoxicity to dendritic cells(DCs)and macrophages.Compared to SiO2 nanoparticles,the microscale vaccine can significantly improve antigen/adjuvant uptake.DCs internal-ized the entire microscale vaccine into lysosomes via macropinocytosis,and an increase in antigen endo/lysosomal escape was observed by confocal laser scanning microscopy(CLSM).Specifically,DCs pulsed with the vaccine were fully mature,expressing high levels of costimulatory molecules(CD40,CD80,and CD86),MHCII,and MHCI and secreting high levels of proinflammatory cytokines(IL-12,TNF-α,IL-1 β,and IL-6).In addition,the vaccine had good in vivo biocompatibility,could protect the antigen from rapid degradation,and increased the retention time in lymph nodes.SiO2 nanoparticles-in-CaCO3 microparticles were an excellent carrier for antigen and adjuvant delivery.Hopefully,this study can provide some information on the design of microscale carriers for vaccine delivery systems.

Rong Xu;Ying Dong;Yajing Zhang;Xiaoli Wang;Chuangnian Zhang;Yanjun Jiang

Tianjin Key Laboratory of Biomaterial Research,Institute of Biomedical Engineering,Chinese Academy of Medical Sciences&Peking Union Medical College,Tianjin 300192,China;School of Chemical Engineering and Technology,Hebei University of Technology,Tianjin,300130,China;NHC Key Laboratory of Hormones and Development,Tianjin Key Laboratory of Metabolic Diseases,Chu Hsien-I Memorial Hospital&Tianjin Institute of Endocrinology,Tianjin Medical University,Tianjin 300134,China

颗粒学报（英文版）

[1]Rong Xu;Ying Dong;Yajing Zhang;Xiaoli Wang;Chuangnian Zhang;Yanjun Jiang-.Programmed nanoparticle-loaded microparticles for effective antigen/adjuvant delivery)[J].颗粒学报（英文版）,2022(01):77-89

MHCII

Programmed,loaded,microparticles,effective,antigen,adjuvant,delivery,microscale,vaccine,containing,SiO2,nanoparticles,CaCO3,was,constructed,using,precipitation,method,ovalbumin,OVA,covalently,conjugated,these,CpG,were,encapsulated,into,generating,size,approximately,Scanning,electron,microscopy,energy,dispersive,ray,EDX,elemental,mapping,Fourier,transform,infrared,FTIR,analyses,confirmed,successful,preparation,had,good,storage,stability,without,sustained,release,negligible,cytotoxicity,dendritic,cells,DCs,macrophages,Compared,significantly,improve,uptake,internal,ized,entire,lysosomes,via,macropinocytosis,endo,lysosomal,escape,observed,by,confocal,laser,scanning,CLSM,Specifically,pulsed,mature,expressing,high,levels,costimulatory,molecules,CD40,CD80,CD86,secreting,proinflammatory,cytokines,In,addition,vivo,biocompatibility,could,protect,from,rapid,degradation,increased,retention,lymph,nodes,excellent,Hopefully,this,study,provide,information,design,carriers,systems

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