Male-specific wing spots are usually associated with wing displays in the courtship behavior of Drosophila and may play important roles in sexual selection.Two closely related species,D.nepalensis and D.trilutea,differ in wing spots and scissor-ing behavior.Here,we compare male morphological characters,pigmentation intensity of male wing spots,wing-scissoring behavior,courtship songs,and reproductive isolation be-tween 2 species.Fl fertile females and sterile males result from the cross between females of D.nepalensis and males of D.trilutea.The pigmentation of wing spots is significantly weaker in D.trilutea than in D.nepalensis and the Fl hybrid.Males scissor both wings in front of the female during courtship,with a posture spreading wings more widely,and at a faster frequency in D.nepalensis than in D.trilutea and the F1s.Males of D.trilutea vibrate wings to produce 2 types(A and B)of pulse songs,whereas D.nepalensis and the F1s sing only type B songs.The incidence of wing vibration and scissoring during courtship suggests that wing vibration is essential but scissoring is a facultative courtship element for successful mating in both species.The association between the darker wing spots with more elaborate scissoring might be the consequence of correlated evolution of these traits in D.nepalensis;however,D.trilutea retains wing scissoring during courtship despite having weaker pigmentation of wing spots.The genetic architecture of 2 traits dif-fers in the F1s,consistent with maternal or sex-linked effects for spots but nonadditive effects for scissoring.

Wen-Zhou Mo;Zhuo-Miao Li;Xiang-Mei Deng;Ai-Li Chen;Michael G.Ritchie;De-Jun Yang;Zhuo-Bin He;Masanori J.Toda;Shuo-Yang Wen

Department of Entomology,College of Plant Protection,South China Agricultural University,Guangzhou,China;School of Biology,University of St Andrews,St Andrews,UK;Acoustics Laboratory,Guangdong Institute of Metrology,South China National Centre of Metrology,Guangzhou,China;The Hokkaido University Museum,Sapporo,Japan

中国昆虫科学（英文版）

[1]Wen-Zhou Mo;Zhuo-Miao Li;Xiang-Mei Deng;Ai-Li Chen;Michael G.Ritchie;De-Jun Yang;Zhuo-Bin He;Masanori J.Toda;Shuo-Yang Wen-.Divergence and correlated evolution of male wing spot and courtship display between Drosophila nepalensis and D.trilutea)[J].中国昆虫科学（英文版）,2022(05):1445-1460

trilutea,scissoring,F1s,vibrate,darker,nonadditive

Divergence,correlated,evolution,courtship,between,Drosophila,nepalensis,specific,spots,usually,associated,displays,behavior,may,important,roles,sexual,selection,Two,closely,species,differ,Here,compare,morphological,characters,pigmentation,intensity,songs,reproductive,isolation,Fl,fertile,females,sterile,result,from,cross,significantly,weaker,than,hybrid,Males,both,wings,front,during,posture,spreading,more,widely,faster,frequency,produce,types,pulse,whereas,sing,only,incidence,vibration,suggests,that,essential,but,facultative,element,successful,mating,association,elaborate,might,consequence,these,traits,however,retains,despite,having,genetic,architecture,fers,consistent,maternal,linked,effects

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