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典型文献
Appraisal of MsepCSP14 for chemosensory functions in Mythimna separata
文献摘要:
Chemosensory proteins (CSPs) have great contributions in performing di-verse functions in insects.However,physiological appraisal of chemosensory protein genes still remains elusive in insects.We studied expression patterns and binding affini-ties of MsepCSP14,a chemosensory protein,in Mythimna separata.The distinct func-tions of MsepCSP14 were validated by employing different molecular techniques.The MsepCSP 14 had high resemblance of sequence with chemosensory proteins of other in-sect family members.The MsepCSP14 expression was higher in antennal tissues of fe-males than other tissues.Fluorescence binding assay validated that binding of nine out of 21 ligands to MsepCSP14 was higher at pH 7.4 than at pH 5.0.Three dimensional modeling (3D) and docking analysis predicted that amino acid residues of MsepCSP14 were involved in binding of compounds,and behavior assay displayed that adults of M.separata considerably responded to four volatiles from compounds demonstrating strong binding ability to MsepCSP14.Results of the present study suggest that MsepCSP14 is likely to mediate chemosensory functions in M.separata.
文献关键词:
作者姓名:
Aneela Younas;Muhammad Irfan Waris;Muhammad Shaaban;Muhammad Tahir ul Qamar;Man-Qun Wang
作者机构:
College of Plant Science and Technology,Huazhong Agricultural University,Wuhan,China;Department of Soil Science,Faculty of Agricultural Sciences and Technology,Bahauddin Zakariya University,Multan,Pakistan;State Key Laboratory of Conservation and Utilization of Subtropical Agro-bioresources,College of Life Science and Technology,Guangxi University,Nanning,530005,China
引用格式:
[1]Aneela Younas;Muhammad Irfan Waris;Muhammad Shaaban;Muhammad Tahir ul Qamar;Man-Qun Wang-.Appraisal of MsepCSP14 for chemosensory functions in Mythimna separata)[J].中国昆虫科学(英文版),2022(01):162-176
A类:
MsepCSP14,affini,MsepCSP
B类:
Appraisal,chemosensory,functions,Mythimna,separata,Chemosensory,proteins,CSPs,have,great,contributions,performing,verse,insects,However,physiological,appraisal,genes,still,remains,elusive,We,studied,expression,patterns,binding,ties,distinct,were,validated,by,employing,different,molecular,techniques,had,resemblance,sequence,other,family,members,was,higher,antennal,tissues,males,than,Fluorescence,assay,that,nine,out,ligands,Three,dimensional,modeling,docking,analysis,predicted,amino,acid,residues,involved,compounds,behavior,displayed,adults,considerably,responded,four,volatiles,from,demonstrating,strong,ability,Results,present,study,suggest,likely,mediate
AB值:
0.516565
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