典型文献
ISG20 inhibits bluetongue virus replication
文献摘要：
ISG20 is an interferon-inducible exonuclease that inhibits virus replication.Although ISG20 is thought to degrade viral RNA,the antiviral mechanism and specificity of ISG20 remain unclear.In this study,the antiviral role of ovine ISG20(oISG20)in bluetongue virus(BTV)infection was investigated.It was found that BTV infection up-regulated the transcription of ovine ISG20(oISG20)in a time-and BTV multiplicity of infection(MOI)-dependent manner.Overexpression of oISG20 suppressed the production of BTV genome,proteins,and virus titer,whereas the knockdown of oISG20 increased viral replication.oISG20 was found to co-localize with BTV proteins VP4,VP5,VP6,and NS2,but only directly interacted with VP4.Exonuclease defective oISG20 significantly decreased the inhibitory effect on BTV replication.In addition,the interaction of mutant oISG20 and VP4 was weakened,suggesting that binding to VP4 was associated with the inhibition of BTV replication.The present data charac-terized the anti-BTV effect of oISG20,and provides a novel clue for further exploring the inhibition mechanism of double-stranded RNA virus by ISG20.
文献关键词：
中图分类号：
作者姓名：
Di Kang;Shandian Gao;Zhancheng Tian;Guorui Zhang;Guiquan Guan;Guangyuan Liu;Jianxun Luo;Junzheng Du;Hong Yin
作者机构：
State Key Laboratory of Veterinary Etiological Biology,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou,730046,China;Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses,Yangzhou University,Yangzhou,225009,China
文献出处：
引用格式：
[1]Di Kang;Shandian Gao;Zhancheng Tian;Guorui Zhang;Guiquan Guan;Guangyuan Liu;Jianxun Luo;Junzheng Du;Hong Yin-.ISG20 inhibits bluetongue virus replication)[J].中国病毒学,2022(04):521-530
A类：
ISG20,exonuclease,oISG20,VP5,Exonuclease
B类：
inhibits,bluetongue,virus,replication,interferon,inducible,that,Although,thought,degrade,antiviral,mechanism,specificity,remain,unclear,In,this,study,role,ovine,BTV,infection,was,investigated,It,found,regulated,transcription,multiplicity,MOI,dependent,manner,Overexpression,suppressed,production,genome,proteins,titer,whereas,knockdown,increased,co,localize,VP4,VP6,NS2,but,only,directly,interacted,defective,significantly,decreased,inhibitory,effect,addition,interaction,mutant,weakened,suggesting,binding,associated,inhibition,present,data,charac,terized,provides,novel,clue,further,exploring,double,stranded,by
AB值：
0.437337
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