典型文献
Human cytomegalovirus RNA2.7 inhibits RNA polymerase Ⅱ(Pol Ⅱ)Serine-2 phosphorylation by reducing the interaction between Pol Ⅱ and phosphorylated cyclin-dependent kinase 9(pCDK9)
文献摘要：
Human cytomegalovirus(HCMV)is a ubiquitous pathogen belongs to betaherpesvirus subfamily.RNA2.7 is a highly conserved long non-coding RNA accounting for more than 20％of total viral transcripts.In our study,functions of HCMV RNA2.7 were investigated by comparison of host cellular transcriptomes between cells infected with HCMV clinical strain and RNA2.7 deleted mutant.It was demonstrated that RNA polymerase Ⅱ(Pol Ⅱ)-dependent host gene transcriptions were significantly activated when RNA2.7 was removed during infection.A 145 nt-in-length motif within RNA2.7 was identified to inhibit the phosphorylation of Pol Ⅱ Serine-2(Pol ⅡS2)by reducing the interaction between Pol Ⅱ and phosphorylated cyclin-dependent kinase 9(pCDK9).Due to the loss of Pol Ⅱ S2 phosphorylation,cellular DNA pre-replication complex(pre-RC)factors,including Cdt1 and Cdc6,were significantly decreased,which prevented more cells from entering into S phase and facilitated viral DNA repli-cation.Our results provide new insights of HCMV RNA2.7 functions in regulation of host cellular transcription.
文献关键词：
中图分类号：
作者姓名：
Yujing Huang;Xin Guo;Jing Zhang;Jianming Li;Mingyi Xu;Qing Wang;Zhongyang Liu;Yanping Ma;Ying Qi;Qiang Ruan
作者机构：
Virology Laboratory,Shengjing Hospital of China Medical University,Shenyang,110004,China;Department of Pediatrics,Shengjing Hospital of China Medical University,Shenyang,110004,China;Department of Obstetrics and Gynecology,Shengjing Hospital of China Medical University,Shenyang,110004,China;Department of Pediatrics,The Fourth Affiliated Hospital,China Medical University,Shenyang,110033,China
文献出处：
引用格式：
[1]Yujing Huang;Xin Guo;Jing Zhang;Jianming Li;Mingyi Xu;Qing Wang;Zhongyang Liu;Yanping Ma;Ying Qi;Qiang Ruan-.Human cytomegalovirus RNA2.7 inhibits RNA polymerase Ⅱ(Pol Ⅱ)Serine-2 phosphorylation by reducing the interaction between Pol Ⅱ and phosphorylated cyclin-dependent kinase 9(pCDK9))[J].中国病毒学,2022(03):358-369
A类：
pCDK9,betaherpesvirus,Cdt1,Cdc6
B类：
Human,cytomegalovirus,RNA2,inhibits,polymerase,Pol,Serine,phosphorylation,by,reducing,interaction,between,phosphorylated,cyclin,dependent,kinase,HCMV,ubiquitous,pathogen,belongs,subfamily,highly,conserved,coding,accounting,more,than,total,viral,transcripts,In,our,study,functions,were,investigated,comparison,host,cellular,transcriptomes,cells,infected,clinical,strain,deleted,mutant,It,was,demonstrated,that,gene,transcriptions,significantly,activated,when,removed,during,infection,length,motif,within,identified,S2,Due,loss,replication,complex,RC,factors,including,decreased,which,prevented,from,entering,into,phase,facilitated,Our,results,provide,new,insights,regulation
AB值：
0.494506
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