典型文献
LncRNA FPASL suppresses fibroblast proliferation through its DNA methylation via DNMT3b in hypertrophic scar
文献摘要：
Long noncoding RNAs(IncRNAs)are increasingly being implicated as key regulators of cell proliferation,apoptosis,and differentiation.However,the molecular mechanisms of specific IncRNAs in the context of hypertrophic scar remain largely unclear.Here,we find that the IncRNA FPASL(fibroblast proliferation-associated LncRNA)is downregulated in HS,and FPASL reduces fibroblast proliferation and colony formation and blocks cell cycle pro-gression.Using GO annotation enrichment analysis along with AZC(a specific inhibitor of DNA methylation),we identify that DNA methylation is responsible for downregulating FPASL in hypertrophic scar.Subsequent studies demonstrate that high expression of DNMT3b inhibits FPASL expression in HS.Mechanistic study reveals a sig-nificant increase in fibroblast proliferation after transfection with LNA-FPASL,which is further inhibited by knock-down of DNMT3b.Thus,our study reveals that DNMT3b mediates hypermethylation of the IncRNA FPASL promoter and the downregulation of IncRNA FPASL promotes fibroblast proliferation in hypertrophic scar.
文献关键词：
中图分类号：
作者姓名：
Kai Wu;Fang Ma;Jiangyong Shen;Hui Zhang;Yu Wan;Xi He;Anning Yang;Jiantuan Xiong;Yun jiao;Zhigang Bai;Shengchao Ma;Yideng Jiang;Huiping Zhang;Yinju Hao
作者机构：
NHC Key Laboratory of Metabolic Cardiovascular Diseases Research,Ningxia Medical University,Yinchuan 750004,China;Ningxia Key Laboratory of Vascular Injury and Repair Research,Ningxia Medical University,Yinchuan 750004,China;School of Basic Medical Sciences,Ningxia Medical University,Yinchuan 750004,China;General Hospital of Ningxia Medical University,Yinchuan 750004,China;Clinical Medical School,Ningxia Medical University,Yinchuan 750004,China;People's Hospital of Ningxia Hui Autonomous Region,Yinchuan 750004,China;Maternal and Child Health of Hunan Province,Changsha 410008,China
文献出处：
引用格式：
[1]Kai Wu;Fang Ma;Jiangyong Shen;Hui Zhang;Yu Wan;Xi He;Anning Yang;Jiantuan Xiong;Yun jiao;Zhigang Bai;Shengchao Ma;Yideng Jiang;Huiping Zhang;Yinju Hao-.LncRNA FPASL suppresses fibroblast proliferation through its DNA methylation via DNMT3b in hypertrophic scar)[J].生物化学与生物物理学报（英文版）,2022(12):1854-1862
A类：
FPASL
B类：
LncRNA,suppresses,fibroblast,proliferation,through,via,DNMT3b,hypertrophic,scar,Long,noncoding,IncRNAs,are,increasingly,being,implicated,key,regulators,cell,apoptosis,differentiation,However,molecular,mechanisms,specific,context,remain,largely,unclear,Here,find,that,associated,downregulated,HS,reduces,colony,formation,blocks,cycle,gression,Using,annotation,enrichment,analysis,along,AZC,inhibitor,identify,responsible,downregulating,Subsequent,studies,demonstrate,high,expression,inhibits,Mechanistic,study,reveals,sig,nificant,increase,after,transfection,LNA,which,further,inhibited,by,knock,Thus,our,mediates,hypermethylation,promoter,downregulation,promotes
AB值：
0.51056
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