Microbes play an important role in the carbon cycle and nutrient flow of the soil ecosystem.However,the response of microbial activities to long-term warming over decades is poorly understood.To determine how warming changes ecoenzyme activity and microbial nutrient limitation,we conducted a long-term,21 years,experiment,on the Qinghai-Tibet Plateau.We selected typical grass-and shrub-covered plots,used fiberglass open-top chambers(OTCs)to raise the temperature,conducted soil sampling at different depths,studied the response of nutrient-acquiring enzyme activity and stoichiometry,and conducted vector analysis of stoichiometry.Our results showed that long-term warming did not have a notable effect on the activity of nutrient-acquiring enzymes or enzymatic stoichiometry.However,Spearman correlation analysis indicated a significant and positive correlation between ecoenzyme activity and the available nutrients and microbial biomass in soil.Vector analysis of stoichiometry showed phosphorus limitation for all soil microbes at different depths,regardless of whether the soil experienced warming.These changes in enzymatic stoichiometry and vector analysis suggested that microbial nutrient limitation was not alleviated substantially by long-term warming,and warming did not considerably affect the stratification of microbial nutrient limitation.Our research has also shown that long-term warming does not significantly change soil ecoenzyme activity and original microbial nutrient limitation at different soil depths within the OTUs'impact range.These results could help improve understanding of microbial thermal acclimation and response to future long-term global warming.

Yuanze Li;Huakun Zhou;Wenjing Chen;Yang Wu;LeiLei Qiao;ZiRan Yan;GuoBin Liu;Sha Xue

State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau,Institute of Soil and Water Conservation,Northwest A&F University,Yangling 712100,China;Qinghai Provincial Key Laboratory of Restoration Ecology in Cold Regions,Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining 810000,China;State Key Laboratory of Plateau Ecology and Agriculture,Qinghai University,Xining 810000,China;Institute of Soil and Water Conservation,Chinese Academy of Sciences and Ministry Water Resources,Yangling 712100,China;College of Natural Resources and Environment,Northwest A&F University,Yangling 712100,China

土壤生态学快报（英文）

[1]Yuanze Li;Huakun Zhou;Wenjing Chen;Yang Wu;LeiLei Qiao;ZiRan Yan;GuoBin Liu;Sha Xue-.Long-term warming does not affect soil ecoenzyme activity and original microbial nutrient limitation on the Qinghai-Tibet Plateau)[J].土壤生态学快报（英文）,2022(04):383-398

ecoenzyme,OTCs

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