Objective: To investigate the effects of non-permeable cryoprotectant, cholesterol-loaded cyclodextrin, when added at different concentrations into cooled and frozen-thawed semen extended with Tris-citrate-fructose egg yolk glycerol and lecithin-based extenders.Methods: A total of 40 ejaculates from four buffalo bulls were collected using artificial vagina. Ejaculates were extended with one of Tris-citrate-fructose egg yolk glycerol and lecithin-based extenders which contained different concentrations [0 (control), 0.75, 1.50, 2.25 and 3.00 mg/mL] of cholesterol-loaded cyclodextrin. The extended semen samples were cooled to 5 ℃ and then frozen slowly to -196 ℃ in 0.25 mL ministraws before being stored in liquid nitrogen pending its evaluation. Sperm motility, live sperm, normal sperm morphology, sperm membrane integrity and acrosome morphology were measured. Results: Supplementation of cholesterol-loaded cyclodextrin improved progressive motility, viability, morphology and acrosome as well as plasma membrane integrities at 1.50-2.25 mg/mL depending upon types of used extenders and stages of pre-and post-freezing process (P<0.01). The best concentration was 1.50 mg/mL at pre-freeze stage and 2.25 mg/mL at post-freezing. However, greater concentration (3.00 mg/mL) of cholesterol-loaded cyclodextrin had a detrimental effect compared to the control group with the two evaluated extenders (P<0.01). Conclusions: Cholesterol-loaded cyclodextrin supplementation at 1.50-2.25 mg/mL concentration could improve pre-frozen and post-thawed buffalo sperm quality. The most suitable concentration is 1.50 mg/mL at pre-freeze stage and 2.25 mg/mL at post-freezing.

Asmaa A.Mostafa;Mohamed S.El-Belely;Sayed T.Ismail;Reda I.El-Sheshtawy;Mohamed I.Shahba

Abassia Frozen Semen Center,General Organization for Veterinary Services,Cairo,Egypt;Department of Theriogenology,Faculty of Veterinary Medicine,Cairo University,Giza,Egypt;Department of Animal Reproduction and Artificial Insemination,National Research Centre,Dokki,Giza,Egypt

亚太生殖杂志（英文版）

[1]Asmaa A.Mostafa;Mohamed S.El-Belely;Sayed T.Ismail;Reda I.El-Sheshtawy;Mohamed I.Shahba-.Effect of cholesterol-loaded cyclodextrin enriched extenders on the quality of pre-frozen and frozen buffalo semen)[J].亚太生殖杂志（英文版）,2022(03):146-152

extenders,buffalo,cryoprotectant,bulls,Ejaculates,ministraws,integrities

Effect,cholesterol,loaded,cyclodextrin,enriched,quality,pre,frozen,semen,Objective,To,investigate,effects,permeable,when,added,different,concentrations,into,cooled,thawed,extended,Tris,citrate,fructose,egg,yolk,glycerol,lecithin,Methods,total,ejaculates,from,four,were,collected,using,artificial,vagina,one,which,contained,control,samples,then,slowly,before,being,stored,liquid,nitrogen,its,evaluation,Sperm,motility,live,sperm,normal,morphology,membrane,integrity,acrosome,measured,Results,Supplementation,improved,progressive,viability,well,plasma,depending,upon,types,used,stages,post,freezing,process,best,was,freeze,However,greater,had,detrimental,compared,group,two,evaluated,Conclusions,Cholesterol,supplementation,could,most,suitable

0.4343