典型文献
The transcription factor Sox30 is involved in Nile tilapia spermatogenesis
文献摘要：
Spermatogenesis is a complex process in which spermatogonial stem cells differentiate and develop into mature spermatozoa.The transcriptional regulatory network involved in fish spermatogenesis remains poorly understood.Here,we demonstrate in Nile tilapia that the Sox transcription factor family member Sox30 is specifically expressed in the testes and mainly localizes to spermatocytes and spermatids.CRISPR/Cas9-mediated sox30 mutation results in abnormal spermiogenesis,reduction of sperm motility,and male subfertility.Comparative transcriptome analysis shows that sox30 mutation alters the expression of genes involved in spermatogenesis.Further chromatin immunoprecipitation followed by high-throughput sequencing(ChIP-seq),ChIP-PCR,and luciferase reporter assays revealed that Sox30 positively regulates the transcription of ift140 and ptprb,two genes involved in spermiogenesis,by directly binding to their promoters.Our data,taken together,indicate that Sox30 plays an essential role in Nile tilapia spermato-genesis by directly regulating the transcription of the spermiogenesis-related genes ift140 and ptprb.
文献关键词：
中图分类号：
作者姓名：
Ling Wei;Yaohao Tang;Xianhai Zeng;Yueqin Li;Song Zhang;Li Deng;Lingsong Wang;Deshou Wang
作者机构：
Key Laboratory of Freshwater Fish Reproduction and Development(Ministry of Education),Key Laboratory of Aquatic Science of Chongqing,School of Life Sciences,Southwest University,Chongqing 400715,China
文献出处：
引用格式：
[1]Ling Wei;Yaohao Tang;Xianhai Zeng;Yueqin Li;Song Zhang;Li Deng;Lingsong Wang;Deshou Wang-.The transcription factor Sox30 is involved in Nile tilapia spermatogenesis)[J].遗传学报,2022(07):666-676
A类：
Sox30,sox30,subfertility,ift140,ptprb,spermato
B类：
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AB值：
0.522061
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