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典型文献
Plastome characteristics and species identification of Chinese medicinal wintergreens(Gaultheria,Ericaceae)
文献摘要:
Wintergreen oil is a folk medicine widely used in foods,pesticides,cosmetics and drugs.In China,nine out of 47 species within Gaultheria(Ericaceae)are traditionally used as Chinese medicinal wintergreens;however,phylogenetic approaches currently used to discriminating these species remain unsatisfactory.In this study,we sequenced and characterized plastomes from nine Chinese wintergreen species and identified candidate DNA barcoding regions for Gaultheria.Each Gaultheria plastome contained 110 unique genes(76 protein-coding,30 tRNA,and four rRNA genes).Duplication of trnfM,rpsl4,and rpl23 genes were detected,while all plastomes lacked ycfl and ycf2 genes.Gaultheria plastomes shared sub-stantially contracted SSC regions that contained only the ndhF gene.Moreover,plastomes of Gaultheria leucocarpa var.yunnanensis contained an inversion in the LSC region and an IR expansion to cover the ndhF gene.Multiple rearrangement events apparently occurred between the Gaultheria plastomes and those from several previously reported families in Ericales.Our phylogenetic reconstruction using 42 plastomes revealed well-supported relationships within all nine Gaultheria species.Additionally,seven mutational hotspot regions were identified as potential DNA barcodes for Chinese medicinal winter-greens.Our study is the first to generate complete plastomes and describe the structural variations of the complicated genus Gaultheria.In addition,our findings provide important resources for identification of Chinese medicinal wintergreens.
文献关键词:
作者姓名:
Yan-Ling Xu;Hao-Hua Shen;Xin-Yu Du;Lu Lu
作者机构:
School of Pharmaceutical Sciences and Yunnan Key Laboratory of Pharmacology for Natural Products,Kunming Medical University,Kunming,Yunnan,China;Germplasm Bank of Wild Species,Kunming Institute of Botany,Chinese Academy of Sciences,Kunming,Yunnan,China
引用格式:
[1]Yan-Ling Xu;Hao-Hua Shen;Xin-Yu Du;Lu Lu-.Plastome characteristics and species identification of Chinese medicinal wintergreens(Gaultheria,Ericaceae))[J].植物多样性(英文),2022(06):519-529
A类:
Plastome,wintergreens,Gaultheria,Wintergreen,wintergreen,plastome,trnfM,rpsl4,ycfl,leucocarpa,Ericales,greens
B类:
characteristics,species,identification,Chinese,medicinal,Ericaceae,oil,folk,medicine,widely,used,foods,pesticides,cosmetics,drugs,In,China,nine,out,within,traditionally,however,phylogenetic,approaches,currently,discriminating,these,remain,unsatisfactory,this,study,sequenced,characterized,plastomes,from,identified,candidate,barcoding,regions,Each,contained,unique,genes,protein,tRNA,four,rRNA,Duplication,rpl23,were,detected,while,lacked,ycf2,shared,sub,stantially,contracted,SSC,that,only,ndhF,Moreover,yunnanensis,inversion,LSC,expansion,cover,Multiple,rearrangement,events,apparently,occurred,between,those,several,previously,reported,families,Our,reconstruction,using,revealed,well,supported,relationships,Additionally,seven,mutational,hotspot,potential,barcodes,first,generate,complete,describe,structural,variations,complicated,genus,addition,findings,provide,important,resources
AB值:
0.488195
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