Anaerobic digestion is widely applied in organic wastewater treatment coupled with bioenergy production,and how to stabilize its work at the high organic loading rate(OLR)remains a challenge Herein,we proposed a new strategy to address this issue via involving the synergetic role of the Aspergillus sydowii 8L-9-F02 immobilized beads(AEBs).A long-term(210-day)continuous-mode operation indicated that the upflow anaerobic sludge bed(UASB)reactor(R1,with AEBs added)could achieve the OLR as high as 25.0 kg/(m3×d),whereas the control reactor(R0,with AEBs free)could only tolerate the maximum OLR of 13.3 kg/(m×d).Remarkably,much higher COD removal(85.9％vs 23.9％)and methane production(5.4 m3/(m3×d)vs 2.2 m3/(m3×d))were achieved in R1 than R0 at the OLR of 25.0 kg/(m3×d).Such favorable effect results from the facts that fungi inhibit VFAs accumulation,favor the pH stabilization,promote the generation of more extracellular polymeric substance,and enhance the sludge granulation and settleability.Moreover,fungi may enhance the secretion of acetyl-coenzyme A,a key compound in converting organic matters to CO2.In addition,fungi are favorable to enrich methanogenic archaea even at high OLR,improving the activity of acetate kinase and coenzyme F420 for more efficient methanogenic pathway.This work may shed new light on how to achieve higher OLR and methane production in anaerobic digestion of wastewater.

Qiong Guo;Zhichao Yang;Bingliang Zhang;Ming Hua;Changhong Liu;Bingcai Pan

State Key Laboratory of Pollution Control and Resource Reuse,School of Environment,Nanjing University,Nanjing 210023,China;Research Center for Environmental Nanotechnology(ReCENT),Nanjing University,Nanjing 210023,China;School of Life Sciences,Nanjing University,State Key Laboratory of Pharmaceutical Biotechnology,Nanjing 210023,China

环境科学与工程前沿

[1]Qiong Guo;Zhichao Yang;Bingliang Zhang;Ming Hua;Changhong Liu;Bingcai Pan-.Enhanced methane production during long-term UASB operation at high organic loads as enabled by the immobilized Fungi)[J].环境科学与工程前沿,2022(06):47-59

AEBs,upflow,settleability

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