The growing interest in the anaerobic ammonium oxidizing(AMX)process in treating high nitrogen containing wastewaters and a comprehensive study into the granulation mecha-nism of these bacteria under diverse environmental conditions over the years have been unequal.To this effect,the distinctive differences in saline adapted AMX(S-AMX)and non-saline adapted AMX(NS_AMX)granules are presented in this study.It was observed that substrate utilisation profiles,granule formation mechanism,and pace towards granulation differed marginally for the two adaptation conditions.The different microbial dominant aggregation types aided in splitting the 471 days operated lab-scale SBRs into three distinct phases.In both reactors,phase Ⅲ(granules dominant phase)showed the highest average nitrogen removal efficiency of 87.9％±4.8％and 85.6％±3.6％for the S-AMX and NS_AMX processes,respectively.The extracellular polymeric substances(EPS)quantity and major composition determined its role either as a binding agent in granulation or a survival mech-anism in saline adaptation.It was also observed that granules of the S_AMX reactor were mostly loosely and less condensed aggregates of smaller sub-units and flocs while those of the NS_AMX reactor were compact agglomerates.The ionic gradient in saline enrich-ment led to an increased activity of the Na+/K+-ATPase,hence enriched granules produced higher cellular adenosine triphosphate molecules which finally improved the granules ac-tive biomass ratio by 32.96％.Microbial community showed that about three to four major known AMX species made up the granules consortia in both reactors.Proteins and expres-sion of functional genes differed for these different species.

Victory Fiifi Dsane;Sumin An;Younggyun Choi

Department of Environmental Engineering,Chungnam National University,Daejeon 305-764,Korea;Department of Food Process Engineering,University of Ghana,Legon,Ghana;Department of Environmental&IT Convergence Engineering,Chungnam National University,Daejeon 305-764,Korea

环境科学学报（英文版）

[1]Victory Fiifi Dsane;Sumin An;Younggyun Choi-.Distinctive differences in the granulation of saline and non-saline enriched anaerobic ammonia oxidizing(AMX)bacteria)[J].环境科学学报（英文版）,2022(12):162-173

SBRs

Distinctive,differences,granulation,saline,enriched,anaerobic,ammonia,oxidizing,AMX,bacteria,growing,interest,ammonium,treating,nitrogen,containing,wastewaters,comprehensive,study,into,these,under,diverse,environmental,conditions,over,years,have,been,unequal,To,this,effect,distinctive,adapted,NS,granules,are,presented,It,observed,that,substrate,utilisation,profiles,formation,mechanism,pace,towards,differed,marginally,two,adaptation,different,microbial,dominant,aggregation,types,aided,splitting,days,operated,lab,scale,three,phases,In,both,reactors,showed,highest,average,removal,efficiency,processes,respectively,extracellular,polymeric,substances,EPS,quantity,major,composition,determined,role,either,binding,agent,survival,also,were,mostly,loosely,less,condensed,aggregates,smaller,units,flocs,while,those,compact,agglomerates,ionic,gradient,led,increased,activity,Na+,K+,ATPase,hence,produced,higher,adenosine,triphosphate,molecules,which,finally,improved,biomass,ratio,by,Microbial,community,about,four,known,species,made,up,consortia,Proteins,expres,sion,functional,genes

0.557508