Background: The aim of the study was to demonstrate the efficacy of human muscle stem cells (MuSCs) isolated using innovative technology in restoring internal urinary sphincter function in a preclinical animal model. Methods: Colonies of pure human MuSCs were obtained from muscle biopsy speci-mens. Athymic rats were subjected to internal urethral sphincter damage by electro-cauterization. Five days after injury, 2 × 105 muscle stem cells or medium as control were injected into the area of sphincter damage ( n = 5 in each group). Peak bladder pressure and rise in pressure were chosen as outcome measures. To repeatedly obtain the necessary pressure values, telemetry sensors had been implanted into the rat bladders 10 days prior to injury. Results: There was a highly significant improvement in the ability to build up peak pressure as well as a pressure rise in animals that had received muscle stem cells as compared to control ( p = 0.007) 3 weeks after the cells had been injected. Only mini-mal histologic evidence of scarring was observed in treated rats. Conclusion: Primary human muscle stem cells obtained using innovative technology functionally restore internal urethral sphincter function after injury. Translation into use in clinical settings is foreseeable.

Biniam M.Bekele;Verena Sch?wel-Wolf;Janine Kieshauer;Andreas Marg;Andreas Busjahn;Sarah Davis;Gayle Nugent;Anne-Karoline Ebert;Simone Spuler

Muscle Research Unit,Experimental and Clinical Research Center,acooperation between the Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association and the Charité,Universit?tsmedizin Berlin,Berlin,Germany;Charité Universit?tsmedizin,Berlin,Germany;Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association,Berlin,Germany;Berlin Institute of Health at Charité Universit?tsmedizin,Belrin,Germany;Health Twist GmbH,Berlin,Germany;Charles River Laboratories,Mattawan,Michigan,USA;Department of Urology and Pediatric Urology,University Hospital Ulm,Ulm,Germany

动物模型与实验医学（英文）

[1]Biniam M.Bekele;Verena Sch?wel-Wolf;Janine Kieshauer;Andreas Marg;Andreas Busjahn;Sarah Davis;Gayle Nugent;Anne-Karoline Ebert;Simone Spuler-.Human primary muscle stem cells regenerate injured urethral sphincter in athymic rats)[J].动物模型与实验医学（英文）,2022(05):453-460

MuSCs,Colonies,Athymic,cauterization

Human,primary,muscle,stem,cells,regenerate,injured,urethral,sphincter,athymic,rats,Background,aim,study,was,demonstrate,efficacy,human,isolated,using,innovative,technology,restoring,internal,urinary,preclinical,model,Methods,pure,were,obtained,from,biopsy,speci,mens,subjected,damage,by,electro,Five,days,after,injury,medium,control,injected,into,area,each,group,Peak,pressure,rise,chosen,outcome,measures,To,repeatedly,necessary,values,telemetry,sensors,had,been,implanted,bladders,prior,Results,There,highly,significant,improvement,ability,build,peak,well,animals,that,received,compared,weeks,Only,mini,histologic,evidence,scarring,observed,treated,Conclusion,Primary,functionally,restore,Translation,use,settings,foreseeable

0.539425