典型文献
Validation of a preclinical dry eye model in New Zealand white rabbits during and following topical instillation of 1％ ophthalmic atropine sulfate
文献摘要：
Background : The objective of this study was to validate an animal model for dry eye during and after the administration of 1％ ophthalmic atropine sulfate (OAS) in New Zealand white (NZW) rabbits. Methods : OAS (1％) was applied three times per day to 30 eyes of 15 healthy NZW rabbits. Sacrifice, enucleation, and lacrimal gland removal took place on days 15, 21, and 30 (OAS group). A second group (n = 5) was used as control. Clinical evalua-tions took place on days 3, 10, 15, 18, 21, 24 and 30. The primary endpoints were: Schirmer I test, tear break- up time (TBUT), and corneal fluorescein staining. As sec-ondary endpoints, clinical changes including intraocular pressure, and histopathology were evaluated. Results : While OAS was administered, the Schirmer I test showed a statistically signif-icant reduction for OAS group versus control ( p < 0.001), and versus basal production ( p < 0.001). TBUT showed statistically significant differences between groups (days 3 and 10; p = 0.001) and versus basal values (day 3; p < 0.001). Fluorescein staining showed a statistically significant difference (day 3; p = 0.001). The most frequent clinical finding was conjunctival hyperemia (76.9％ OAS vs. 20％ control). For histo-pathology, all OAS subjects presented some degree of inflammation (86.7％ minimal; 13.3％ mild) whereas the control presented only 30％ minimal inflammation. Goblet cell density showed no difference. Conclusions : The effectiveness of the OAS dry eye model in NZW rabbits as reported in previous studies was confirmed, provided that the application of the drug is main-tained throughout the intervention; it is not a viable model after OAS administration is suspended.
文献关键词：
中图分类号：
作者姓名：
Alejandra Sánchez-Ríos;Elba Yadira Correa-Gallegos;JoséManuel Medina-Espinoza;Andrea Anaid Navarro-Sanchez;Oscar Olvera-Monta?o;Leopoldo Baiza-Durán;Patricia Mu?oz-Villegas
作者机构：
Medical Affairs Department,Laboratorios Sophia,SA de CV,Zapopan,Jalisco, Mexico
文献出处：
引用格式：
[1]Alejandra Sánchez-Ríos;Elba Yadira Correa-Gallegos;JoséManuel Medina-Espinoza;Andrea Anaid Navarro-Sanchez;Oscar Olvera-Monta?o;Leopoldo Baiza-Durán;Patricia Mu?oz-Villegas-.Validation of a preclinical dry eye model in New Zealand white rabbits during and following topical instillation of 1％ ophthalmic atropine sulfate)[J].动物模型与实验医学（英文）,2022(03):266-273
A类：
NZW,Sacrifice,Goblet
B类：
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AB值：
0.494415
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