Objective:This study aims to investigate the effect of ω-3 fatty acid immunonutritional therapy on natural killer(NK)cell gene methylation and function in elderly patients with gastric cancer.Methods:A total of 70 cases of elderly patients with gastric cancer were randomized into the ω-3 fatty acid group and placebo group,according to the type of nutritional support administered.The methylation status of the tumor necrosis factor(TNF)-α gene promoter in peripheral NK cells was detected by methylation specific polymerase chain reaction,and the TNF-αlevel in peripheral NK cells was detected by enzyme-linked immunosorbent assay.Results:After 14 days of immunonutritional therapy with co-3 fatty acid or placebo,patients in the ω-3 group had significantly higher average NK cell activity(0.27 vs.0.24,P=0.013)and lower percentages of TNF-α gene promoter methylation(25.7％vs.60％,P＜0.05)than the placebo group.However,there were no significant differences in the concentration of albumin,prealbumin and TNF-α in serum,and the NK cell count between the ω-3 group and placebo group.Conclusion:The postoperative application of ω-3 fatty acid may improve the activity of NK cells,which is correlated to the methylation status of the TNF-α gene promoter.

Li-ning XU;Ying-ying XU;Gui-ping LI;Bo YANG

Department of General Surgery,The Second Medical Center&National Clinical Research Center for Geriatric Diseases,Chinese PLA General Hospital,Beijing 100853,China;Department of Internal Medicine,Henan Cancer Hospital,Zhengzhou 450003,China;Department of Radiology,Hubei Province Integrated Hospital of Chinese and Western Medicine,Wuhan 430015,China;Department of Radiology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China

当代医学科学(英文)

[1]Li-ning XU;Ying-ying XU;Gui-ping LI;Bo YANG-.Effect of Postoperative ω-3 Fatty Acid Immunonutritional Therapy on NK Cell Gene Methylation in Elderly Patients with Gastric Cancer)[J].当代医学科学(英文),2022(02):373-378

Immunonutritional,immunonutritional

Effect,Postoperative,Fatty,Acid,Therapy,NK,Cell,Gene,Methylation,Elderly,Patients,Gastric,Cancer,Objective,This,study,aims,investigate,effect,fatty,acid,therapy,natural,killer,gene,methylation,function,elderly,patients,gastric,cancer,Methods,total,cases,were,randomized,into,group,placebo,according,type,support,administered,status,tumor,necrosis,promoter,peripheral,cells,was,detected,by,specific,polymerase,chain,reaction,level,enzyme,linked,immunosorbent,assay,Results,After,days,had,significantly,higher,average,activity,lower,percentages,than,However,there,differences,concentration,prealbumin,serum,count,between,Conclusion,postoperative,application,may,improve,which,correlated

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