典型文献
Genetic Characteristics of Lipooligosaccharide and Capsular Polysaccharide of Campylobacter jejuni from Different Sources in China
文献摘要：
Objective To determine the distribution of two important virulence factors [lipooligosaccharide (LOS) and capsular polysaccharide (CPS)] in Campylobacter jejuni (C. jejuni) isolated from different sources in China and to develop a rapid screening method for Guillain–Barré syndrome (GBS)-associated strains. Methods Whole-genome sequencing was carried out for 494 C. jejuni strains. The OrthoMCL software was used to define the LOS/CPS gene clusters. CPS genotyping was performed with serotype-specific sequence alignment using the BLAST software. Real-time Polymerase chain reaction (PCR) was developed with the unique sequences of specific CPS types. Results Nine novel and 29 previously confirmed LOS classes were identified. LOS classes A, B, and C were the most common (48.2％, 238/494) among the 494 strains. Twenty-six capsular types were identified in 448 strains. HS2, HS4c, HS5/31, HS19, and HS8/17 were the most frequent CPS genotypes (58.7％, 263/448). Strains of 17 CPS genotypes (strain number > 5) had one or two prevalent LOS classes (P < 0.05). Multiplex real-time PCR for rapid identification of HS2, HS19, and HS41 was developed and validated with strains of known serotypes. Conclusion Our results describe the genetic characteristics of the important virulence factors in C. jejuni strains in China. The multiplex real-time PCR developed in this study will facilitate enhanced surveillance of GBS-associated strains in China.
文献关键词：
中图分类号：
作者姓名：
WANG Jia Qi;CHEN Xiao Li;ZHOU Gui Lan;WANG Hai Rui;GU Yi Xin;ZHANG Jian Zhong;SHAO Zhu Jun;ZHANG Mao Jun
作者机构：
State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable DiseaseControl and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China;NationalInstitute of Environmental Health, Chinese Center for Disease Control and Prevention, Beijing 100021, China
文献出处：
引用格式：
[1]WANG Jia Qi;CHEN Xiao Li;ZHOU Gui Lan;WANG Hai Rui;GU Yi Xin;ZHANG Jian Zhong;SHAO Zhu Jun;ZHANG Mao Jun-.Genetic Characteristics of Lipooligosaccharide and Capsular Polysaccharide of Campylobacter jejuni from Different Sources in China)[J].生物医学与环境科学（英文版）,2022(12):1106-1114
A类：
Lipooligosaccharide,lipooligosaccharide,OrthoMCL,HS4c,HS19,HS41
B类：
Genetic,Characteristics,Capsular,Polysaccharide,Campylobacter,jejuni,from,Different,Sources,China,Objective,To,determine,distribution,two,important,virulence,factors,LOS,capsular,polysaccharide,CPS,isolated,different,sources,rapid,screening,method,Guillain,Barr,syndrome,GBS,associated,strains,Methods,Whole,genome,sequencing,was,carried,out,software,used,define,clusters,genotyping,performed,specific,alignment,using,BLAST,Real,Polymerase,chain,reaction,developed,unique,sequences,Results,Nine,novel,previously,confirmed,classes,were,identified,most,common,among,Twenty,six,HS2,HS5,HS8,frequent,genotypes,Strains,number,had,one,prevalent,Multiplex,real,identification,validated,known,serotypes,Conclusion,Our,results,describe,genetic,characteristics,multiplex,this,study,will,facilitate,enhanced,surveillance
AB值：
0.520081
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