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典型文献
Chloroplast gene matK holds the barcodes for identification of Momordica (Cucurbitaceae) species from Indian subcontinent
文献摘要:
DNA barcoding is a supplementary tool in plant systematics, extensively used to resolve the species level controversies. This paper details the identification of DNA barcodes for seven species of Momordica, using the chloroplast gene matK. Since the species M. cymbalaria has been confused as a member of the genus Luffa, 26 accessions of Momordica belonging to seven Indian species and two accessions of Luffa acutangula were included in this study. Analysis of matK sequences has yielded distinct barcodes in M. charantia var. charantia, M. subangulata subsp. renig-era, M. cochinchinensis, M. balsamina, M. cymbalaria and also in Luffa acutangula. Evolutionary status of each species was reflected as nucleotide polymorphisms in each sequence. The wild species M. dioica and M. sahyadrica have yielded one barcode but failed to get differentiated. Further, this study provides conclusive proof that M. cymbalaria is a member of Momordica genus. The phylogram generated was successful to distin-guish the monoecious species of this genus, M. charantia, M. balsamina and M. cymbalaria, from the dioecious species M. dioica, M. sahyadrica, M. subangulata subsp. renigera and M. cochinchinensis. Thus, matK locus, by accumulating the evolutionary sequence variations, is proven efficient to differentiate the Momordica species and to reveal their relatedness.
文献关键词:
作者姓名:
Girme Aoudumbar Ramesh;Deepu Mathew;K.Joseph John;V.Ravisankar
作者机构:
Centre for Plant Biotechnology and Molecular Biology,Kerala Agricultural University,Thrissur,Kerala State 680656,India;Bioinformatics Centre,Kerala Agricultural University,Thrissur,Kerala State,680656,India;ICAR-National Bureau of Plant Genetic Resources,Regional Station,Thrissur,Kerala State 680656,India
引用格式:
[1]Girme Aoudumbar Ramesh;Deepu Mathew;K.Joseph John;V.Ravisankar-.Chloroplast gene matK holds the barcodes for identification of Momordica (Cucurbitaceae) species from Indian subcontinent)[J].园艺学报(英文版),2022(01):89-98
A类:
cymbalaria,Luffa,acutangula,subangulata,renig,dioica,sahyadrica,phylogram,guish,monoecious,renigera
B类:
Chloroplast,matK,holds,barcodes,identification,Momordica,Cucurbitaceae,species,from,Indian,subcontinent,barcoding,supplementary,tool,plant,systematics,extensively,resolve,level,controversies,This,paper,details,seven,using,chloroplast,Since,has,been,confused,member,genus,accessions,belonging,two,were,included,this,study,Analysis,sequences,yielded,distinct,charantia,subsp,cochinchinensis,balsamina,also,Evolutionary,status,each,was,reflected,nucleotide,polymorphisms,wild,have,one,but,failed,get,differentiated,Further,provides,conclusive,proof,that,generated,successful,dioecious,Thus,locus,by,accumulating,evolutionary,variations,proven,efficient,reveal,their,relatedness
AB值:
0.435556
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