Diabetes melllitus(DM)has emerged as a serious public health concern,due to the high morbidity and mortality resulted from its complications,such as diabetic nephropathy,diabetic cardiovascular complication,and diabetic neuropathy,etc.In this study,we investigated the beneficial effects of sepia ink melanin(SIM)on hyperglycaemia and the restoration of diabetic symptoms in streptozotocin(STZ)-induced diabetic model mice.At first,the normal experimental mice were performed with intraperitoneal injec-tion of STZ(40 mg(kg BW)-1)(BW,body weight)to attain diabetes and then were treated with different concentrations of SIM(120,240 and 480 mg(kg BW)-1)for four weeks.After treatment,significant decrease in gluconeogenesis were determined,accompanied by a notable increase in both glycolysis and oxidative enzyme activities in SIM-treated groups,such as liver marker enzymes in the serum and key antioxidant enzymes in liver.qPCR results revealed the transcriptional alterations in SIM-treated groups.SIM exposure in-creased the expression levels of several genes related to insulin transduction and PI3K/Akt pathway,including PI3K,Akt,Irs-2,and InsR.Meanwhile,expression levels of Dicarbonyl/1-xylulose reductase(Dcxr)and UDP-glucose dehydrogenase(Ugdh),which are in-volved in pentose-glucuronate interconversion pathway,were also elevated in SIM-treated groups.Furthermore,histological observation results indicated that nuclear deformation and organelle dissolution were improved,thus could enhance the liver function.These results demonstrated that SIM can be effective in ameliorating diabetic symptoms and improving disease management for diabetic patients.

WANG Xiaopeng;HAN Meng;DONG Hui;ZHAO Yun;SONG Weiwei;WANG Chunlin;MU Changkao;LI Ronghua

Key Laboratory of Applied Marine Biotechnology,Ministry of Education,Ningbo University,Ningbo 315832,China;Collaborative Innovation Center for Zhejiang Marine High-Efficiency and Healthy Aquaculture,Ningbo 315832,China

中国海洋大学学报（自然科学英文版）

[1]WANG Xiaopeng;HAN Meng;DONG Hui;ZHAO Yun;SONG Weiwei;WANG Chunlin;MU Changkao;LI Ronghua-.Protective Effects of Sepia Ink Melanin on Hepatic Tissue in Streptozotocin-Induced Diabetic Mice)[J].中国海洋大学学报（自然科学英文版）,2022(05):1362-1372

melllitus,sepia,Irs,Dicarbonyl,Dcxr,Ugdh

Protective,Effects,Sepia,Ink,Melanin,Hepatic,Tissue,Streptozotocin,Induced,Diabetic,Mice,Diabetes,DM,has,emerged,serious,public,health,concern,due,high,morbidity,mortality,resulted,from,its,complications,such,diabetic,nephropathy,cardiovascular,neuropathy,etc,this,study,investigated,beneficial,effects,ink,melanin,SIM,hyperglycaemia,restoration,symptoms,streptozotocin,STZ,induced,model,mice,At,first,normal,experimental,were,performed,intraperitoneal,injec,BW,body,weight,attain,diabetes,then,treated,different,concentrations,four,weeks,After,treatment,significant,decrease,gluconeogenesis,determined,accompanied,by,notable,increase,both,glycolysis,oxidative,activities,groups,liver,marker,enzymes,serum,key,antioxidant,qPCR,results,revealed,transcriptional,alterations,exposure,creased,expression,levels,several,related,insulin,transduction,PI3K,Akt,pathway,including,InsR,Meanwhile,xylulose,reductase,UDP,glucose,dehydrogenase,which,are,volved,pentose,glucuronate,interconversion,also,elevated,Furthermore,histological,observation,indicated,that,nuclear,deformation,organelle,dissolution,improved,thus,could,enhance,function,These,demonstrated,effective,ameliorating,improving,disease,management,patients

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