典型文献
A Label-Free Colorimetric Aptasensor Containing DNA Triplex Molecular Switch and AuNP Nanozyme for Highly Sensitive Detection of Saxitoxin
文献摘要：
Saxitoxin(STX),one of the most toxic paralytic shellfish poisons discovered to date,is listed as a required item of aqua-tic product safety inspection worldwide.However,conventional detection methods for STX are limited by various issues,such as low sensitivity,complicated operations,and ethical considerations.In this study,an aptamer-triplex molecular switch(APT-TMS)and gold nanoparticle(AuNP)nanozyme were combined to develop a label-free colorimetric aptasensor for the rapid and highly sensitive de-tection of STX.An anti-STX aptamer designed with pyrimidine arms and a purine chain was fabricated to form an APT-TMS.Spe-cific binding between the aptamer and STX triggered the opening of the switch,which causes the purine chains to adsorb onto the surface of the AuNPs and enhances the peroxidase-like activity of the AuNP nanozyme toward 3,3',5,5'-tetramethylbenzidine.Under optimized conditions,the proposed aptasensor showed high sensitivity and selectivity for STX,with a limit of detection of 335.6 pmol L-1 and a linear range of 0.59-150nmol L-1.Moreover,good recoveries of 82.70％-92.66％for shellfish and 88.97％-106.5％for seawater were obtained.The analysis could be completed within 1 h.The proposed design also offers a robust strategy to achieve detection of other marine toxin targets by altering the corresponding aptamers.
文献关键词：
中图分类号：
作者姓名：
QI Xiaoyan;LI Ling;YAN Xiaochen;ZHAO Yinglin;WANG Lele;MA Rui;WANG Sai;MAO Xiangzhao
作者机构：
College of Food Science and Engineering,Ocean University of China,Qingdao 266003,China;Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology,Qingdao 266237,China
文献出处：
引用格式：
[1]QI Xiaoyan;LI Ling;YAN Xiaochen;ZHAO Yinglin;WANG Lele;MA Rui;WANG Sai;MAO Xiangzhao-.A Label-Free Colorimetric Aptasensor Containing DNA Triplex Molecular Switch and AuNP Nanozyme for Highly Sensitive Detection of Saxitoxin)[J].中国海洋大学学报（自然科学英文版）,2022(05):1343-1350
A类：
Colorimetric,Aptasensor,Triplex,Saxitoxin,aqua,150nmol
B类：
Label,Free,Containing,Molecular,Switch,Nanozyme,Highly,Sensitive,Detection,STX,one,most,toxic,paralytic,shellfish,poisons,discovered,date,listed,required,item,product,safety,inspection,worldwide,However,conventional,detection,methods,are,limited,by,various,issues,such,low,sensitivity,complicated,operations,ethical,considerations,In,this,study,triplex,molecular,switch,APT,TMS,gold,nanoparticle,nanozyme,were,combined,develop,label,free,colorimetric,aptasensor,rapid,highly,sensitive,An,anti,designed,pyrimidine,arms,purine,was,fabricated,form,Spe,cific,binding,between,triggered,opening,which,causes,chains,adsorb,onto,surface,AuNPs,enhances,peroxidase,like,activity,toward,tetramethylbenzidine,Under,optimized,conditions,proposed,showed,selectivity,pmol,linear,range,Moreover,good,recoveries,seawater,obtained,analysis,could,completed,within,also,offers,robust,strategy,achieve,other,marine,targets,altering,corresponding,aptamers
AB值：
0.607744
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