One of the most promising tools for the control of fungal plant diseases is spray-induced gene silencing (SIGS). In SIGS, small interfering RNA (siRNA) or double-stranded RNA (dsRNA) tar-geting essential or virulence-related pathogen genes are exogenously applied to plants and postharvest products to trigger RNA interfer-ence (RNAi) of the targeted genes, inhibiting fungal growth and disease. However, SIGS is limited by the unstable nature of RNA under environmental conditions. The use of layered double hydroxide or clay particles as carriers to deliver biologically active dsRNA, a formulation termed BioClay?, can enhance RNA durability on plants, prolonging its activity against patho-gens. Here, we demonstrate that dsRNA deliv-ered as BioClay can prolong protection against Botrytis cinerea, a major plant fungal pathogen, on tomato leaves and fruit and on mature chickpea plants. BioClay increased the pro-tection window from 1 to 3 weeks on tomato leaves and from 5 to 10 days on tomato fruits, when compared with naked dsRNA. In flowering chickpea plants, BioClay provided prolonged protection for up to 4 weeks, covering the crit-ical period of poding, whereas naked dsRNA provided limited protection. This research rep-resents a major step forward for the adoption of SIGS as an eco-friendly alternative to traditional fungicides.

Jonatan Ni?o-Sánchez;Prabhakaran T.Sambasivam;Anne Sawyer;Rachael Hamby;Angela Chen;Elizabeth Czislowski;Peng Li;Narelle Manzie;Donald M.Gardiner;Rebecca Ford;Zhi Ping Xu;Neena Mitter;Hailing Jin

Department of Microbiology&Plant Pathology,Center for Plant Cell Biology,Institute for Integrative Genome Biology,University of California,RiversideCalifornia,92521,USA;Department of Plant Production and Forest Resources,University of Valladolid,Palencia 34004,Spain;iuFOR-Sustainable Forest Management Research Institute,University of Valladolid–INIA,Palencia 34004,Spain;Centre for Planetary Health and Food Security,Griffith University,Nathan,Queensland,4111,Australia;School of Chemistry and Molecular Biosciences,The University of Queensland,St Lucia,Queensland,4072,Australia;Queensland Alliance for Agriculture and Food Innovation,Centre for Horticultural Science,The University of Queensland,St Lucia,Queensland,4072,Australia;School of Agriculture and Food Sciences,The University of Queensland,St Lucia,Queensland,4072,Australia;Australian Institute for Bioengineering and Nanotechnology,The University of Queensland,St Lucia,Queensland,4072,Australia

植物学报（英文版）

[1]Jonatan Ni?o-Sánchez;Prabhakaran T.Sambasivam;Anne Sawyer;Rachael Hamby;Angela Chen;Elizabeth Czislowski;Peng Li;Narelle Manzie;Donald M.Gardiner;Rebecca Ford;Zhi Ping Xu;Neena Mitter;Hailing Jin-.BioClay?prolongs RNA interference-mediated crop protection against Botrytis cinerea)[J].植物学报（英文版）,2022(11):2187-2198

BioClay,SIGS,poding

prolongs,interference,mediated,crop,protection,against,Botrytis,cinerea,One,most,promising,tools,control,fungal,diseases,spray,induced,silencing,In,small,interfering,siRNA,double,stranded,dsRNA,geting,essential,virulence,related,pathogen,genes,exogenously,applied,plants,postharvest,products,trigger,RNAi,targeted,inhibiting,growth,However,limited,by,unstable,nature,under,environmental,conditions,use,layered,hydroxide,clay,particles,carriers,deliver,biologically,active,formulation,termed,can,enhance,durability,prolonging,activity,gens,Here,demonstrate,that,major,tomato,leaves,mature,chickpea,increased,window,from,weeks,days,fruits,when,compared,naked,flowering,provided,prolonged,up,covering,crit,period,whereas,This,research,rep,resents,step,forward,adoption,eco,friendly,alternative,traditional,fungicides

0.522565