Asian cultivated rice shows allelic variation in sodium transporter, OsHKT1;5, correlating with shoot sodium exclusion (salinity tolerance). These changes map to intra/extracellularly-oriented loops that occur between four transmembrane-P loop-transmembrane (MPM) motifs in OsHKT1;5. HKT1;5 sequences from more recently evolved Oryza species (O. sativa/O. officinalis complex species) contain two expansions that involve two intracellularly oriented loops/helical regions between MPM domains, potentially governing transport characteristics, while more ancestral HKT1;5 sequences have shorter intracellular loops. We compared homology models for homoeologous OcHKT1;5-K and OcHKT1;5-L from halophytic O. coarctata to identify complementary amino acid residues in OcHKT1;5-L that potentially enhance affinity for Na+. Using haplotyping, we showed that Asian cultivated rice accessions only have a fraction of HKT1;5 diversity available in progenitor wild rice species (O. nivara and O. rufipogon). Progenitor HKT1;5 haplotypes can thus be used as novel potential donors for enhancing cultivated rice salinity tolerance. Within Asian rice accessions, 10 non-synonymous HKT1;5 haplotypic groups occur. More HKT1;5 haplotypic diversities occur in cultivated indica gene pool compared to japonica. Predominant Haplotypes 2 and 10 occur in mutually exclusive japonica and indica groups, corresponding to haplotypes in O. sativa salt-sensitive and salt-tolerant landraces, respectively. This distinct haplotype partitioning may have originated in separate ancestral gene pools of indica and japonica, or from different haplotypes selected during domestication. Predominance of specific HKT1;5 haplotypes within the 3000 rice dataset may relate to eco-physiological fitness in specific geo-climatic and/or edaphic contexts.

Shalini PULIPATI;Suji SOMASUNDARAM;Nitika RANA;Kavitha KUMARESAN;Mohamed SHAFI;Peter CIVá?;Gothandapani SELLAMUTHU;Deepa JAGANATHAN;Prasanna Venkatesan RAMARAVI;S.PUNITHA;Kalaimani RAJU;Shrikant S.MANTRI;R.SOWDHAMINI;Ajay PARIDA;Gayatri VENKATARAMAN

M.S.Swaminathan Research Foundation,Chennai 600113,India;National Agri-Food Biotechnology Institute,Punjab 140306,India;Krishi Vigyan Kendra,Kanyakumari 629901,India;National Centre for Biological Sciences/Tata Institute of Fundamental Research,Bangalore 560065,India;National Research Institute for Agriculture,Food and Environment University,Clermont-Auvergne,Clermont-Ferrand 63000,France;Forest Molecular Entomology Laboratory,Faculty of Forestry and Wood Sciences,Czech University of Life Sciences,Prague,Praha 16500,Czech Republic;Tamil Nadu Agricultural University,Coimbatore 641003,India;International Business Machines India,Chennai 600125,India;Institute of Life Sciences,Bhubaneswar 751023,India

水稻科学（英文版）

[1]Shalini PULIPATI;Suji SOMASUNDARAM;Nitika RANA;Kavitha KUMARESAN;Mohamed SHAFI;Peter CIVá?;Gothandapani SELLAMUTHU;Deepa JAGANATHAN;Prasanna Venkatesan RAMARAVI;S.PUNITHA;Kalaimani RAJU;Shrikant S.MANTRI;R.SOWDHAMINI;Ajay PARIDA;Gayatri VENKATARAMAN-.Diversity of Sodium Transporter HKT1;5 in Genus Oryza)[J].水稻科学（英文版）,2022(01):31-46,中插1-中插16

HKT1,OsHKT1,extracellularly,homoeologous,OcHKT1,halophytic,coarctata,haplotyping,nivara,haplotypic,Haplotypes,Predominance

Diversity,Sodium,Transporter,Genus,Oryza,Asian,cultivated,rice,shows,allelic,variation,sodium,transporter,correlating,shoot,exclusion,salinity,tolerance,These,changes,map,oriented,loops,that,occur,between,four,transmembrane,MPM,motifs,sequences,from,more,recently,evolved,species,sativa,officinalis,complex,contain,two,expansions,involve,intracellularly,helical,regions,domains,potentially,governing,characteristics,while,ancestral,have,shorter,We,compared,homology,models,identify,complementary,amino,acid,residues,enhance,affinity,Na+,Using,showed,accessions,only,fraction,diversity,available,progenitor,wild,rufipogon,Progenitor,haplotypes,can,thus,used,novel,donors,enhancing,Within,synonymous,groups,More,diversities,indica,gene,japonica,Predominant,mutually,exclusive,corresponding,salt,sensitive,tolerant,landraces,respectively,This,distinct,partitioning,may,originated,separate,pools,different,selected,during,domestication,specific,within,dataset,relate,eco,physiological,fitness,geo,climatic,edaphic,contexts

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