A narrow genetic base has hindered improvement of Brassica juncea(AjAjBjBj).In this study,large-scale genomic components were introduced from diploid ancestor species into modern B.juncea using a dige-nomic hexaploid strategy.The hexaploids AjAjArArBjBj and AjAjBjBjBnBn were first developed from B.juncea x B.rapa(ArAr)and B.juncea×B.nigra(BnBn),and then crossed with dozens of B.nigra and B.rapa,respectively.Both types of hexaploid showed high pollen fertility and moderate seed set throughout the S1 to S3 generations,and could be crossed with diploid progenitor species under field conditions,in par-ticular for the combination of AjAjBjBjBnBn×B.rapa.Thirty AjArBnBj-type and 31 AjArBnBj-type B.juncea resources were generated,of which the AAjArBnBj type showed higher fertility.Of these new-type B.juncea resources,97 individual plants were genotyped with 42 simple sequence repeat markers,together with 16 current B.juncea accessions and 30 hexaploid plants.Based on 180 polymorphic loci,the new-type B.juncea resources and current B.juncea were separated clearly into distinct groups,with large genetic dis-tance between the new-type B.juncea resources and current B.juncea.Our study provides a novel approach to introducing large-scale genomic components from diploid ancestor species into B.juncea.

Jiaqin Mei;Jin Liu;Fang Yue;Yangui Chen;Jiayi Ming;Zhiyong Xiong;Fengqun Yu;Jiana Li;Wei Qian

College of Agronomy and Biotechnology,Southwest University,Chongqing 400716,China;Academy of Agricultural Sciences,Southwest University,Chongqing 400716,China;State Cultivation Base of Crop Stress Biology for Southern Mountainous Land of Southwest University,Chongqing 400715,China;Key Laboratory of Herbage and Endemic Crop Biotechnology,School of Life Sciences,Inner Mongolia University,Hohhot 010070,Inner Mongolia,China;Agriculture and Agri-Food Canada,Saskatoon Research and Development Center,107 Science Place,Saskatoon,SK S7N 0X2,Canada

作物学报（英文版）

[1]Jiaqin Mei;Jin Liu;Fang Yue;Yangui Chen;Jiayi Ming;Zhiyong Xiong;Fengqun Yu;Jiana Li;Wei Qian-.Broadening the genetic base of Brassica juncea by introducing genomic components from B.rapa and B.nigra via digenomic allohexaploid bridging)[J].作物学报（英文版）,2022(03):672-679

Broadening,digenomic,AjAjBjBj,dige,hexaploids,AjAjArArBjBj,AjAjBjBjBnBn,ArAr,BnBn,AjArBnBj,AAjArBnBj

genetic,base,Brassica,juncea,by,introducing,components,from,rapa,nigra,via,allohexaploid,bridging,narrow,has,hindered,improvement,In,this,study,large,scale,were,introduced,diploid,ancestor,species,into,modern,using,strategy,first,developed,then,crossed,dozens,respectively,Both,types,showed,pollen,fertility,moderate,seed,set,throughout,S1,S3,generations,could,progenitor,under,field,conditions,ticular,combination,Thirty,resources,generated,which,higher,Of,these,new,individual,plants,genotyped,simple,sequence,repeat,markers,together,current,accessions,Based,polymorphic,loci,separated,clearly,distinct,groups,tance,between,Our,provides,novel,approach

0.421477