典型文献
Transcriptome analysis shows nicotinamide seed treatment alters expression of genes involved in defense and epigenetic processes in roots of seedlings of Picea abies
文献摘要：
Nicotinamide treatment of plants and plant cell cultures has been shown to promote defense and decrease levels of DNA methylation. In the present study, we used RNA-seq technology to study overall changes in gene expression induced in roots of 3-month-old spruce ( Picea abies ) seedlings grown from nicotinamide-treated seeds to examine the molecular mechanisms underlying the defense promotion. Approximately 350 genes were identi-fied as differentially expressed in roots after the seed treat-ment. Stress response genes, including transcription factors MYB77 and LHY and two chitinase enzymes, were gen-erally upregulated, whereas genes thought to be involved in epigenetic regulation such as DDM1, known to promote DNA methylation, were present at high frequency among the downregulated genes. Across all samples, the expression of downregulated epigenetic-related genes was highly cor-related with the nicotinamide treatment, indicating a com-mon regulation. Our results support an earlier hypothesis regarding a potential role of nicotinamide as a defense-signal mediator.
文献关键词：
中图分类号：
作者姓名：
Cecilia Laurell;Torkel Berglund;Anna B.Ohlsson
作者机构：
Department of Industrial Biotechnology,School of Engineering Sciences in Chemistry,Biotechnology and Health(CBH),KTH Royal Institute of Technology, 10691 Stockholm,Sweden
文献出处：
引用格式：
[1]Cecilia Laurell;Torkel Berglund;Anna B.Ohlsson-.Transcriptome analysis shows nicotinamide seed treatment alters expression of genes involved in defense and epigenetic processes in roots of seedlings of Picea abies)[J].林业研究（英文版）,2022(04):1365-1375
A类：
abies,MYB77,LHY,DDM1
B类：
Transcriptome,analysis,shows,nicotinamide,treatment,alters,expression,genes,involved,defense,epigenetic,processes,roots,seedlings,Picea,Nicotinamide,plants,cell,cultures,has,been,shown,promote,decrease,levels,methylation,In,present,study,used,seq,technology,overall,changes,induced,month,old,spruce,grown,from,treated,seeds,examine,molecular,mechanisms,underlying,promotion,Approximately,were,identi,fied,differentially,expressed,after,Stress,response,including,transcription,factors,two,chitinase,enzymes,erally,upregulated,whereas,thought,regulation,such,known,frequency,among,downregulated,Across,samples,related,was,highly,cor,indicating,com,Our,results,support,earlier,hypothesis,regarding,potential,role,signal,mediator
AB值：
0.556209
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