典型文献
Inhibition of miR397 by STTM technology to increase sweetpotato resistance to SPVD
文献摘要：
As a critical food crop, sweetpotato (Ipomoea batatas (L.) Lam.) is widely planted all over the world, but it is deeply affected by Sweetpotato Virus Disease (SPVD). The present study utilized short tandem target mimic (STTM) technology to effectively up-regulate the expression of laccase (IbLACs) by successfully inhibiting the expression of miR397. The upstream genes in the lignin synthesis pathway were widely up-regulated by feedback regulation, including phenylalanine ammonialyase (PAL), 4-coumarate-CoAligase (4CL), hydroxycinnamoyl CoA:shikimatetransferase (HTC), caffeicacid O-methyltransferase (COMT), and cinnamyl alcohol dehydrogenase (CAD). Meanwhile, the activities of PAL and LAC increased significantly, finally leading to increased lignin content. Lignin deposition in the cell wall increased the physical defence ability of transgenic sweetpotato plants, reduced the accumulation of SPVD transmitted by Bemisia tabaci (Gennadius), and promoted healthy sweetpotato growth. The results provide new insights for disease resistance breeding and green production of sweetpotato.
文献关键词：
中图分类号：
作者姓名：
LI Chen;LIU Xuan-xuan;ABOUELNASR Hesham;MOHAMED HAMED Arisha;KOU Meng;TANG Wei;YAN Hui;WANG Xin;WANG Xiao-xiao;ZHANG Yun-gang;LIU Ya-ju;GAO Run-fei;MA Meng;LI Qiang
作者机构：
Xuzhou Institute of Agricultural Sciences in Jiangsu Xuhuai District/Sweetpotato Research Institute,Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Breeding of Sweetpotato,Ministry of Agriculture and Rural Affairs,Xuzhou 221131,P.R.China;School of Life Science,Jiangsu Normal University,Xuzhou 221116,P.R.China;Plant Pathology Department,Agriculture and Biology Research Division,National Research Center,Giza 12622,Egypt;Department of Horticulture,Faculty of Agriculture,Zagazig University,Zagazig 44511,Egypt
文献出处：
引用格式：
[1]LI Chen;LIU Xuan-xuan;ABOUELNASR Hesham;MOHAMED HAMED Arisha;KOU Meng;TANG Wei;YAN Hui;WANG Xin;WANG Xiao-xiao;ZHANG Yun-gang;LIU Ya-ju;GAO Run-fei;MA Meng;LI Qiang-.Inhibition of miR397 by STTM technology to increase sweetpotato resistance to SPVD)[J].农业科学学报（英文）,2022(10):2865-2875
A类：
sweetpotato,Sweetpotato,IbLACs,coumarate,CoAligase,hydroxycinnamoyl,shikimatetransferase,caffeicacid,Gennadius
B类：
Inhibition,miR397,by,STTM,technology,resistance,SPVD,critical,food,crop,Ipomoea,batatas,Lam,widely,planted,over,world,but,deeply,affected,Virus,Disease,present,study,utilized,short,tandem,target,mimic,effectively,expression,laccase,successfully,inhibiting,upstream,genes,lignin,synthesis,pathway,were,regulated,feedback,regulation,including,phenylalanine,ammonialyase,PAL,4CL,HTC,methyltransferase,COMT,cinnamyl,alcohol,dehydrogenase,CAD,Meanwhile,activities,increased,significantly,finally,leading,content,Lignin,deposition,cell,wall,physical,defence,ability,transgenic,plants,reduced,accumulation,transmitted,Bemisia,tabaci,promoted,healthy,growth,results,provide,new,insights,disease,breeding,green,production
AB值：
0.586653
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机标中图分类号，由域田数据科技根据网络公开资料自动分析生成，仅供学习研究参考。
