典型文献
miR-99a-5p inhibits target gene FZD5 expression and steroid hormone secretion from goat ovarian granulosa cells
文献摘要：
MicroRNA (miRNA) has vital regulatory effects on the proliferation, differentiation and secretion of ovarian granulosa cells, but the role of miR-99a-5p in goat ovarian granulosa cells (GCs) is unclear. Both miR-99a-5p and Frizzled-5 (FZD5) were found to be expressed in GCs in goat ovaries via fluorescence in situ hybridization and immunohistochemistry, respectively, and FZD5 was verified (P<0.001) as a target gene of miR-99a-5p by double luciferase reporter gene experiments. Furthermore, FZD5 mRNA and protein expression were both found to be regulated (P<0.05) by miR-99a-5p in GCs. Moreover, the overexpression of miR-99a-5p or knockdown of FZD5 suppressed (P<0.05) estradiol and progesterone secretion from the GCs, as determined by ELISA. In summary, miR-99a-5p inhibits target gene FZD5 expression and estradiol and progesterone synthesis in GCs. Our study thus provides seminal data and new insights into the regulatory mechanisms of follicular development in the goat and other animals.
文献关键词：
中图分类号：
作者姓名：
ZHU Lu;JING Jing;QIN Shuai-qi;LU Jia-ni;ZHU Cui-yun;ZHENG Qi;LIU Ya;FANG Fu-gui;LI Yun-sheng;ZHANG Yun-hai;LING Ying-hui
作者机构：
College of Animal Science and Technology,Anhui Agricultural University,Hefei 230036,P.R.China;Local Animal Genetic Resources Conservation and Biobreeding Laboratory of Anhui Province,Hefei 230036,P.R.China
文献出处：
引用格式：
[1]ZHU Lu;JING Jing;QIN Shuai-qi;LU Jia-ni;ZHU Cui-yun;ZHENG Qi;LIU Ya;FANG Fu-gui;LI Yun-sheng;ZHANG Yun-hai;LING Ying-hui-.miR-99a-5p inhibits target gene FZD5 expression and steroid hormone secretion from goat ovarian granulosa cells)[J].农业科学学报（英文）,2022(04):1137-1145
A类：
FZD5
B类：
99a,5p,inhibits,target,gene,steroid,hormone,secretion,from,goat,ovarian,granulosa,cells,MicroRNA,miRNA,has,vital,regulatory,effects,proliferation,differentiation,but,role,GCs,unclear,Both,Frizzled,were,found,be,expressed,ovaries,via,fluorescence,situ,hybridization,immunohistochemistry,respectively,was,verified,by,double,luciferase,reporter,experiments,Furthermore,protein,both,regulated,Moreover,overexpression,knockdown,suppressed,estradiol,progesterone,determined,In,summary,synthesis,Our,study,thus,provides,seminal,data,new,insights,into,mechanisms,follicular,development,other,animals
AB值：
0.462673
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