A well understanding about protein adsorption into charged polymer brushes is of importance in the elu-cidation of mechanism and important phenomena(such as"chain delivery"effect)in protein adsorption on polymer-grafted ion exchange adsorbents.In this work,quartz crystal microbalance with dissipation(QCM-D)was introduced to in situ investigate lysozyme adsorption on QCM sensors grafted with poly(3-sulfopropyl methacrylate)(pSPM)via atom transfer radical polymerization.It was achieved by analyzing frequency(f)and energy dissipation(D)shift simultaneously on pSPM-grafted sensors.The result showed that an initial decrease in ΔD was typical of lysozyme adsorption on pSPM-grafted sensor and more sig-nificant with an increase of chain length and grafting density.It was attributed to significant water release in the hydration layer of protein and polymer chains in lysozyme adsorption into pSPM brushes.On pSPM-grafted sensors with long and dense chains,furthermore,lysozyme transitioned from mono-layer to multilayer adsorption and the maximum adsorbed amount was obtained to be 374.0 ng·cm-2 among all pSPM-grafted sensors in this work.The results in D-f plot further revealed that lysozyme adsorption into pSPM brushes increased the rigidity of adsorbed layer and little structure adjustment of adsorbed lysozyme.It was unfavorable for"chain delivery"effect for facilitated transport of adsorbed protein.This work provided valuable insight into protein adsorption in pSPM brushes and outlined a fea-sible approach to increasing mass transport in polymer-grafted ion exchange adsorbents.

Fenfen You;Qing-Hong Shi

Department of Biochemical Engineering,School of Chemical Engineering and Technology,Tianjin University,Tianjin 300350,China;Key Laboratory of Systems Bioengineering(Ministry of Education),Tianjin University,Tianjin 300072,China

中国化学工程学报（英文版）

[1]Fenfen You;Qing-Hong Shi-.In situ investigation of lysozyme adsorption into polyelectrolyte brushes by quartz crystal microbalance with dissipation)[J].中国化学工程学报（英文版）,2022(08):106-115

elu,cidation,sulfopropyl,pSPM

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