Natural products(NPs)and their structural analogs represent a major source of novel drug development for disease prevention and treatment.The development of new drugs from NPs includes two crucial aspects.One is the discovery of NPs from medicinal plants/microorganisms,and the other is the evaluation of the NPs in vivo at various physiological and pathological states.The heterogeneous spatial distribution of NPs in medicinal plants/microorganisms or In vivo can provide valuable information for drug development.However,few molecular imaging technologies can detect thousands of compounds simultaneously on a label-free basis.Over the last two decades,mass spectrometry imaging(MSI)methods have progressively improved and diversified,thereby allowing for the development of various applications of NPs in plants/microorganisms and in vivo NP research.Because MSI allows for the spatial mapping of the production and distribution of numerous molecules in situ without labeling,it provides a visualization tool for NP research.Therefore,we have focused this mini-review on summarizing the applications of MSI technology in discovering NPs from medicinal plants and evaluating NPs in preclinical studies from the perspective of new drug research and development(R&D).Additionally,we briefly reviewed the factors that should be carefully considered to obtain the desired MSI results,Finally,the future development of MSI in new drug R&D is proposed.

Jin-jun Hou;Zi-jia Zhang;Wen-yong Wu;Qing-qing He;Teng-qian Zhang;Ya-wen Liu;Zhao-jun Wang;Lei Gao;Hua-li Long;Min Lei;Wan-ying Wu;De-an Guo

National Engineering Research Center of TCM Standardization Technology,Shanghai Institute of Materia Medica,Chinese Academy of Sciences,Shanghai 201203,China;University of Chinese Academy of Sciences,Beijing 100049,China;School of Chinese Materia Medica,Nanjing University of Chinese Medicine,Nanjing 210029,China

中国药理学报（英文版）

[1]Jin-jun Hou;Zi-jia Zhang;Wen-yong Wu;Qing-qing He;Teng-qian Zhang;Ya-wen Liu;Zhao-jun Wang;Lei Gao;Hua-li Long;Min Lei;Wan-ying Wu;De-an Guo-.Mass spectrometry imaging:new eyes on natural products for drug research and development)[J].中国药理学报（英文版）,2022(12):3096-3111

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