典型文献
A novel and effective approach to generate germline-like monoclonal antibodies by integration of phage and mammalian cell display platforms
文献摘要：
Phage display technology allows for rapid selection of antibodies from the large repertoire of human antibody fragments displayed on phages.However,antibody fragments should be converted to IgG for biological characterizations and affinity of antibodies obtained from phage display library is frequently not sufficient for efficient use in clinical settings.Here,we describe a new approach that combines phage and mammalian cell display,enabling simultaneous affinity screening of full-length IgG antibodies.Using this strategy,we successfully obtained a novel germline-like anti-TIM-3 monoclonal antibody named m101,which was revealed to be a potent anti-TIM-3 therapeutic monoclonal antibody via in vitro and in vivo experiments,indicating its effectiveness and power.Thus,this platform can help develop new monoclonal antibody therapeutics with high affinity and low immunogenicity.
文献关键词：
中图分类号：
作者姓名：
Yu-jia Jin;Diao Yu;Xiao-long Tian;Hui-xian Li;Xiao-chao Zhou;Yu Kong;Wei Zhang;Lu Zhang;Cheng Lei;Zhen-lin Yang;Chao Tu;Yan-ling Wu;Tian-lei Ying
作者机构：
MOE/NHC Key Laboratory of Medical Molecular Virology,Shanghai Institute of Infectious Disease and Biosecurity,School of Basic Medical Sciences,Shanghai Medical College,Fudan University,Shanghai 200032,China;Biomissile Corporation,Shanghai 201203,China;Department of Pulmonary Medicine,Zhongshan Hospital,Fudan University,Shanghai 200032,China
文献出处：
引用格式：
[1]Yu-jia Jin;Diao Yu;Xiao-long Tian;Hui-xian Li;Xiao-chao Zhou;Yu Kong;Wei Zhang;Lu Zhang;Cheng Lei;Zhen-lin Yang;Chao Tu;Yan-ling Wu;Tian-lei Ying-.A novel and effective approach to generate germline-like monoclonal antibodies by integration of phage and mammalian cell display platforms)[J].中国药理学报（英文版）,2022(04):954-962
A类：
m101
B类：
novel,approach,generate,germline,like,monoclonal,antibodies,by,integration,mammalian,cell,platforms,Phage,technology,allows,rapid,selection,from,large,repertoire,human,antibody,fragments,displayed,phages,However,should,converted,IgG,biological,characterizations,affinity,obtained,library,frequently,not,sufficient,efficient,use,clinical,settings,Here,describe,new,that,combines,enabling,simultaneous,screening,length,Using,this,strategy,successfully,TIM,named,which,was,revealed,potent,via,vitro,vivo,experiments,indicating,its,effectiveness,power,Thus,can,help,develop,therapeutics,high,immunogenicity
AB值：
0.56528
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