Biogenic synthesis of silver nanoparticles(b-AgNPs)utilising plant extract has gained the interest of researchers due to the environmentally friendly and cost-effective technique.However,the extent of its application in the biomedical field remains scarce.This study evaluates the antibacterial,cytocompatibil-ity,and wound healing activities of synthesised AgNPs using Persicaria odorata leaves extract(PO-AgNPs).The formation of PO-AgNPs was observed by visual colour changes and verified by ultraviolet-visible(UV-vis)spectrophotometer,which revealed a surface plasmon resonance(SPR)at around 440 nm,and further confirmed by X-ray diffraction(XRD).Characterisation using Fourier transform infrared(FTIR)spectroscopy showed biomolecules from the leaves extract presented together on PO-AgNPs.Field emission scanning electron microscope(FESEM)and high-resolution transmission electron microscopy(HR-TEM)images revealed PO-AgNPs nanospheres with diameters of 11±3 nm.Disc diffusion test(DDT)and minimum inhibitory concentration(MIC)analysis resulted in a dose-dependent inhibition of PO-AgNPs against tested Staphylococcus epidermidis and Methicillin-resistant Staphylococcus aureus(MRSA).These results were further corroborated by time-kill kinetic assay which revealed that PO-AgNPs were bactericidal against both strains 24 h post-treatment.Cytocompatibility and in vitro wound healing eval-uation against normal human fibroblast cells,HSF 1184 inferred that PO-AgNPs are non-toxic to normal cells and able to enhance cell migration as compared to the non-treated cells.Therefore,PO-AgNPs are biocompatible and possess antibacterial and wound healing capabilities that are useful in biomedical applications.

Faridah Aminullah Lubis;Nik Ahmad Nizam Nik Malek;Nor Suriani Sani;Khairunadwa Jemon

Department of Biosciences,Faculty of Science,Universiti Teknologi Malaysia,81310 UTM,Skudai,Johor,Malaysia;Centre for Sustainable Nanomaterials(CSNano),Ibnu Sina Institute for Scientific and Industrial Research(ISI-ISIR),Universiti Teknologi Malaysia,81310 UTM,Skudai,Johor,Malaysia;Office of Deputy Vice Chancellor(Research and Innovation),Universiti Teknologi Malaysia,81310 UTM,Skudai,Johor,Malaysia

颗粒学报（英文版）

[1]Faridah Aminullah Lubis;Nik Ahmad Nizam Nik Malek;Nor Suriani Sani;Khairunadwa Jemon-.Biogenic synthesis of silver nanoparticles using Persicaria odorata leaf extract:Antibacterial,cytocompatibility,and in vitro wound healing evaluation)[J].颗粒学报（英文版）,2022(11):10-19

cytocompatibil,Cytocompatibility

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