典型文献
The actions of neonicotinoid insecticides on nicotinic acetylcholine subunits Ldα1 and Ldα8 of Leptinotarsa decemlineata and assembled receptors
文献摘要：
The insect nicotinic acetylcholine receptor(nAChR)is a pentameric channel protein and also a target for neonicotinoids.There are few reported studies on the molecular interactions of Leptinotarsa decemlineata nAChRs with neonicotinoids.In this study,we analyzed the response of acetylcholine and neonicotinoids(thiamethoxam[TMX],imidacloprid[IMI],and clothianidin[CLO])on hybrid receptors constructed by nAChR α1 and α8 subunits of L.decemlineata(Ldα 1 and Ldα8)co-expressed with rat β2 subunit(rβ2)at different capped RNA(cRNA)ratios in Xenopus oocytes.In addition,we evaluated the expression changes of Ldα1 andLdα8 after median lethal dose of TMX treat-ment for 72 h by quantitative polymerase chain reaction(qPCR).The resulting functional nAChRs Ldα1/rβ2 and Ldα1/Ldα8/rβ2 showed different pharmacological characteristics.The neonicotinoids tested showed lower agonist affinity on Ldα1/Ldα8/rβ2 compared to Ldα1/rβ2 at same ratios of subunit cRNAs.The sensitivities of neonicotinoids tested for Ldα1/rβ2 and Ldα1/Ldα8/rβ2 at cRNA ratios of 5∶1,1∶1 and 5∶5∶1,1∶1∶1,respectively,were lower than those for nAChRs at ratios of 1∶5 and 1∶1∶5,respectively,whereas the val-ues of maximum response(Imax)varied.For Ldα1/Ldα8/rβ2,a reduction of Lda8 cRNA resulted in increased sensitivity to IMI and decreased sensitivity to TMX.The expression of Ldα1 and Ldα8 significantly decreased in adults by 82.12％and 47.02％,respectively,while Ldα8 was significantly upregulated by 2.44 times in 4th instar larvae after exposure to TMX.We infer that Ldα1 and Ldα 8 together play an important role in the sensitivity of L.decemlineata to neonicotinoids.
文献关键词：
中图分类号：
作者姓名：
Zhi-Min Wang;Sha Li;Cheng-Cheng Shi;Lin-Jie Xie;Kai-Yun Fu;Wei-Hua Jiang
作者机构：
College of Plant Protection,Nanjing Agricultural UniversitylKey Laboratory of Integrated Management of Crop Disease and Pests,Ministry of EducationlKey Laboratory of Integrated Pest Management on Crops in East China,Ministry of Agriculture,Nanjing 210095,China;Institute of Plant Protection,Xinjiang Academy of Agricultural Sciences/Key Laboratory of Integrated Pest Management on Crop in Northwestern Oasis,Ministry Agriculture P.R.China,Urumqi,China
文献出处：
引用格式：
[1]Zhi-Min Wang;Sha Li;Cheng-Cheng Shi;Lin-Jie Xie;Kai-Yun Fu;Wei-Hua Jiang-.The actions of neonicotinoid insecticides on nicotinic acetylcholine subunits Ldα1 and Ldα8 of Leptinotarsa decemlineata and assembled receptors)[J].中国昆虫科学（英文版）,2022(05):1387-1400
A类：
Leptinotarsa,decemlineata,pentameric,thiamethoxam,clothianidin,andLd,Lda8
B类：
insecticides,nicotinic,acetylcholine,subunits,assembled,receptors,channel,protein,also,target,neonicotinoids,There,few,reported,studies,molecular,interactions,nAChRs,In,this,study,analyzed,response,TMX,imidacloprid,IMI,CLO,hybrid,constructed,by,expressed,different,capped,ratios,Xenopus,oocytes,addition,evaluated,expression,changes,after,median,lethal,dose,treat,ment,quantitative,polymerase,chain,reaction,qPCR,resulting,functional,showed,pharmacological,characteristics,tested,lower,agonist,affinity,compared,same,cRNAs,sensitivities,respectively,were,than,those,whereas,ues,maximum,Imax,varied,For,reduction,resulted,increased,sensitivity,decreased,significantly,adults,while,was,upregulated,times,4th,instar,larvae,exposure,We,infer,that,together,play,important,role
AB值：
0.396663
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