Insect CAPA neuropeptidesare considered to affect water and ion balance by mediating the physiological metabolism activities of the Malpighian tubules.In previ-ous studies,the CAPA-PK analogue 1895(2Abf-Suc-FGPRLamide)was reported to de-crease aphid fitness when administered through microinjection or via topical application.However,a further statistically significant decrease in the fitness of aphids and an in-creased mortality could not be established with pairwise combinations of 1895 with other CAPA analogue.In this study,we assessed the topical application of new combinations of 1895 with five CAPA-PVK analogues on the fitness of aphids.We found that 1895 and CAPA-PVK analogue 2315(ASG-[β3L]-VAFPRVamide)was statistically the most effec-tive combination to control the peach potato aphid Myzus persicae nymphs via topical application,leading to 72％mortality.Additionally,the combination(1895+2315)was evaluated against a selection of beneficial insects,that is,a pollinator(Bombus terrestris)and three natural enemies(Chrysoperla carnea,Nasonia vitripennis,and Adalia bipunc-tata).We found no significant influence on food intake,weight increase,and survival for the pollinator and the three representative natural enemies.These results could facilitate to further establish and generate CAPA analogues as alternatives to broad spectrum and less friendly insecticides.

Yan Shi;Ronald J.Nachman;Shun-Hua Gui;Niels Piot;Krzysztof Kaczmarek;Janusz Zabrocki;Julian A.T.Dow;Shireen-A.Davies;Guy Smagghe

College of Plant Health and Medicine,Qingdao Agricultural University,Qingdao,Shandong,China;Laboratory of Agrozoology,Depart-ment of Plants and Crops,Faculty of Bioscience Engineering,Ghent University,Ghent,Belgium;U.S.Department of Agriculture,Insect Neuropeptide Laboratory,Insect Control and Cotton Disease Research Unit,Southern Plains Agricultural Research Center,College Sta-tion,Austin,Texas,USA;Institute of Entomology,Guizhou University,Guiyang,Guizhou,China;Institute of Organic Chemistry,Lodz University of Technology,Lodz,90-924,Poland;College of Medical,Veterinary and Life Sciences,Institute of Molecular,Cell and Systems Biology,University of Glasgow,Glasgow,UK

中国昆虫科学（英文版）

[1]Yan Shi;Ronald J.Nachman;Shun-Hua Gui;Niels Piot;Krzysztof Kaczmarek;Janusz Zabrocki;Julian A.T.Dow;Shireen-A.Davies;Guy Smagghe-.Efficacy and biosafety assessment of neuropeptide CAPA analogues against the peach-potato aphid(Myzus persicae))[J].中国昆虫科学（英文版）,2022(02):521-530

neuropeptidesare,Malpighian,2Abf,FGPRLamide,VAFPRVamide,1895+2315,Chrysoperla,Nasonia,vitripennis,Adalia,bipunc,tata

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