Although several artificial nanotherapeutics have been approved for practical treatment of metastatic breast cancer,their inefficient therapeutic outcomes,serious adverse effects,and high cost of mass production remain crucial challenges.Herein,we developed an alternative strategy to specifically trigger apoptosis of breast tumors and inhibit their lung metastasis by using natural nanovehicles from tea flowers(TFENs).These nanovehicles had desirable particle sizes(131 nm),exosome-like morphology,and negative zeta potentials.Furthermore,TFENs were found to contain large amounts of polyphenols,flavonoids,functional proteins,and lipids.Cell experiments revealed that TFENs showed strong cytotox-icities against cancer cells due to the stimulation of reactive oxygen species(ROS)amplification.The increased intracellular ROS amounts could not only trigger mitochondrial damage,but also arrest cell cycle,resulting in the in vitro anti-proliferation,anti-migration,and anti-invasion activities against breast cancer cells.Further mice investigations demonstrated that TFENs after intravenous(i.v.)injection or oral administration could accumulate in breast tumors and lung metastatic sites,inhibit the growth and metas-tasis of breast cancer,and modulate gut microbiota.This study brings new insights to the green produc-tion of natural exosome-like nanoplatform for the inhibition of breast cancer and its lung metastasis via i.v.and oral routes.

Qiubing Chen;Qian Li;Yuqi Liang;Menghang Zu;Nanxi Chen;Brandon S.B.Canup;Liyong Luo;Chenhui Wang;Liang Zeng;Bo Xiao

State Key Laboratory of Silkworm Genome Biology,College of Sericulture,Textile,and Biomass Sciences,Southwest University,Chongqing 400715,China;Chongqing Key Laboratory of Soft-Matter Material Chemistry and Function Manufacturing,School of Materials and Energy,Southwest University,Chongqing 400715,China;Department of Chemistry,Center for Diagnostics and Therapeutics,Georgia State University,Atlanta,GA 30303,USA;College of Food Science,Southwest University,Chongqing 400715,China;Chongqing Key Laboratory of Natural Product Synthesis and Drug Research,School of Pharmaceutical Sciences,Chongqing University,Chongqing 401331,China

药学学报（英文版）

[1]Qiubing Chen;Qian Li;Yuqi Liang;Menghang Zu;Nanxi Chen;Brandon S.B.Canup;Liyong Luo;Chenhui Wang;Liang Zeng;Bo Xiao-.Natural exosome-like nanovesicles from edible tea flowers suppress metastatic breast cancer via ROS generation and microbiota modulation)[J].药学学报（英文版）,2022(02):907-923

nanovehicles,TFENs,cytotox,icities

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