典型文献
Bioluminescence imaging of mouse monocyte chemoattractant protein-1 expression in inflammatory processes
文献摘要：
Monocyte chemoattractant protein-1(MCP-1)plays a crucial role in various inflammatory diseases.To reveal the impact of MCP-1 during diseases and to develop anti-inflammatory agents,we establish a transgenic mouse line.The firefly luciferase gene is incorporated into the mouse genome and driven by the endogenous MCP-1 promoter.A bioluminescence photographing system is applied to monitor luciferase levels in live mice during inflammation,including lipopolysaccharide-induced sepsis,concanavalin A-induced T cell-dependent liver injury,CCI4-induced acute hepatitis,and liver fibrosis.The results demonstrate that the luciferase signal induced in inflammatory processes is correlated with endogenous MCP-1 expression in mice.Furthermore,the expressions of MCP-1 and the luciferase gene are dramatically inhibited by administration of the anti-inflammatory drug dexamethasone in a septicemia model.Our results suggest that the transgenic MCP-1-Luc mouse is a useful model to study MCP-1 expression in inflammation and disease and to evaluate the efficiency of anti-inflammatory drugs in vivo.
文献关键词：
中图分类号：
作者姓名：
Fangyang Shao;Lei Ci;Jiahao Shi;Fei Fang;Bowen Yan;Xijun Liu;Xiangyu Yao;Mengjie Zhang;Hua Yang;Zhugang Wang;Jian Fei
作者机构：
School of Life Sciences and Technology,Tongji University,Shanghai 200092,China;Institute of Biophysics,Chinese Academy of Sciences,Beijing 100101,China;College of Life Sciences,University of Chinese Academy of Sciences,Beijing 100049,China;Shanghai Engineering Research Center for Model Organisms,SMOC,Shanghai 201203,China
文献出处：
引用格式：
[1]Fangyang Shao;Lei Ci;Jiahao Shi;Fei Fang;Bowen Yan;Xijun Liu;Xiangyu Yao;Mengjie Zhang;Hua Yang;Zhugang Wang;Jian Fei-.Bioluminescence imaging of mouse monocyte chemoattractant protein-1 expression in inflammatory processes)[J].生物化学与生物物理学报（英文版）,2022(10):1507-1517
A类：
photographing
B类：
Bioluminescence,imaging,mouse,monocyte,chemoattractant,protein,inflammatory,processes,Monocyte,MCP,plays,crucial,role,various,diseases,To,reveal,impact,during,develop,anti,agents,we,establish,transgenic,line,firefly,luciferase,gene,incorporated,into,genome,driven,by,endogenous,promoter,bioluminescence,system,applied,monitor,levels,mice,inflammation,including,lipopolysaccharide,induced,sepsis,concanavalin,cell,dependent,liver,injury,CCI4,acute,hepatitis,fibrosis,results,demonstrate,that,signal,correlated,Furthermore,expressions,are,dramatically,inhibited,administration,dexamethasone,septicemia,model,Our,suggest,Luc,useful,study,evaluate,efficiency,drugs,vivo
AB值：
0.570813
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