The presence of senescent cells is associated with renal fibrosis.This study aims to investigate the effect of albumin-induced premature senescence on tubulointerstitial fibrosis and its possible mechanism in vitro.Different con-centrations of bovine serum albumim(BSA)with or without si-p21 are used to stimulate HK-2 cells for 72 h,and SA-β-gal activity,senescence-associated secretory phenotypes(SASPs),LaminB1 are used as markers of senescence.Immunofluorescence staining is performed to characterize the G2/M phase arrest between the control and BSA groups.Alterations in the DNA damage marker γ-H2AX,fibrogenesis,and associated proteins at the G2/M phase,such as p21,p-CDC25C and p-CDK1,are evaluated.Compared with those in the control group,the SA-β-gal activity,SASP,and γ-H2AX levels are increased in the BSA group,while the level of LaminB1 is decreased.Meanwhile,HK-2 cells blocked at the G2/M phase are significantly increased under the stimulation of BSA,and the levels of p21,p-CDC25C and p-CDK1,as well as fibrogenesis are also increased.When p21 expression is inhibited,the levels of p-CDC25C and p-CDK1 are decreased and the G2/M phase arrest is improved,which decreases the production of fibrogenesis.In conclusion,BSA induces renal tubular epithelial cell premature senescence,which regulates the G2/M phase through the CDC25C/CDK1 pathway,leading to tubulointerstitial fibrosis.

Wen Lu;Shijing Ren;Wenhui Dong;Xiaomin Li;Zongji Zheng;Yijie Jia;Yaoming Xue

Department of Endocrinology and Metabolism,Nanfang Hospital,Southern Medical University,Guangzhou 510000,China

生物化学与生物物理学报（英文版）

[1]Wen Lu;Shijing Ren;Wenhui Dong;Xiaomin Li;Zongji Zheng;Yijie Jia;Yaoming Xue-.Albumin-induced premature senescence in human renal proximal tubular cells and its relationship with intercellular fibrosis)[J].生物化学与生物物理学报（英文版）,2022(07):893-903

albumim,SASPs,LaminB1

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